Table 1 Plasmids and primers used in this study.
Plasmids or primers | Relevant characteristics | Sources or references |
|---|---|---|
Plasmids | Â | Â |
pWL3E | 11.2-kb, phaEHme and its native promoter | [6] |
pWL3EC | 12.6-kb, phaECHme and its native promoter | [6] |
pWL3EC1 | 12.5-kb, derivative of pWL3E, phaC1 insert | This study |
pWL3EC2 | 12.2-kb, derivative of pWL3E, phaC2 insert | This study |
pWL3EC3 | 12.9-kb, derivative of pWL3E, phaC3 insert | This study |
Primers for RT-PCR (5'-3' sequence) | Â | |
phaC-RT-F | GTACATTCTCGACCTCCA | Â |
phaC-RT-R | AGGAACGTTTCCAAACGC | Â |
phaC1-RT-F | ATGACCATGAACCCGTTT | Â |
phaC1-RT-R | CGATGTACCGATTCACGT | Â |
phaC2-RT-F | ATGTTCCCTAACCTCGTC | Â |
phaC2-RT-R | GTCCGCCTCAGTTCGTTC | Â |
phaC3-RT-F | ATGACAGATGATGGGCGT | Â |
phaC3-RT-R | CGATGTAGCGATTGACGT | Â |
Primers for expression (5'-3' sequence a ) | ||
phaC1-BamHI-F | ATAGGATCCATGACCATGAACCCGTTT | Â |
phaC1-KpnI-R | AGCGGTACCTTAGCTGGTGCGTTGTCT | Â |
phaC2-BamHI-F | ATAGGATCCATGTTCCCTAACCTCGTC | Â |
phaC2-KpnI-R | AGCGGTACCTTATCCGTTTGGTGACGC | Â |
phaC3-BamHI-F | ATAGGATCCATGACAGATGATGGGCGT | Â |
phaC3-KpnI-R | AGCGGTACCTCAGTCATCTTCTGTACC | Â |