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On the origin of prokaryotic "species": the taxonomy of halophilic Archaea
Saline Systems volume 4, Article number: 5 (2008)
The consistent use of the taxonomic system of binomial nomenclature (genus and species) was first popularized by Linnaeus nearly three-hundred years ago to classify mainly plants and animals. His main goal was to give labels that would ensure that biologists could agree on which organism was under investigation. One-hundred fifty years later, Darwin considered the term species as one of convenience and not essentially different from variety. In the modern era, exploration of the world's niches together with advances in genomics have expanded the number of named species to over 1.8 million, including many microorganisms. However, even this large number excludes over 90% of microorganisms that have yet to be cultured or classified. In naming new isolates in the microbial world, the challenge remains the lack of a universally held and evenly applied standard for a species. The definition of species based on the capacity to form fertile offspring is not applicable to microorganisms and 70% DNA-DNA hybridization appears rather crude in light of the many completed genome sequences. The popular phylogenetic marker, 16S rRNA, is tricky for classification since it does not provide multiple characteristics or phenotypes used classically for this purpose. Using most criteria, agreement may usually be found at the genus level, but species level distinctions are problematic. These observations lend credence to the proposal that the species concept is flawed when applied to prokaryotes. In order to address this topic, we have examined the taxonomy of extremely halophilic Archaea, where the order, family, and even a genus designation have become obsolete, and the naming and renaming of certain species has led to much confusion in the scientific community.
An important challenge in the classification of microorganisms is ensuring that scientists can follow the pedigree of isolates in the literature. This laudable goal is however especially difficult to achieve for microbes that have a long history and where variants have been isolated and re-isolated from an abundance of niches in the laboratories of many different investigators. For example, the group of extremely salt-loving halophilic microbes which produce red, pink, and purple hues in hypersaline ponds used to make salt from the sea, were among the earliest microorganisms to be recognized and described. They were, not surprisingly, originally identified as agents of food spoilage, before the advent of refrigeration when salting was widely used for preserving fish and meats . Some early isolates from dried and salted codfish (Klippfisch) were documented in a 1919 review on causative agents of fish reddening by the German botanist, Klebahn . Notably, he isolated and named "Bacillus halobius ruber", aware that these bright vermillion halophilic microbes were not spore formers, but his isolate was subsequently lost. A dozen years later, halophilic isolates thought to be similar to Klebahn's, were named "Bacterium halobium" by Petter in the Kluyver laboratory in Delft, Holland . In the 1940s–1960s, additional halophilic microorganisms were isolated from different countries and reported in the scientific literature with names such as Halobacterium halobium, H. salinarium, and H. cutirubrum . Many of these isolates were deposited in US, Canadian, and European culture collections, but a substantial number of them were subsequently lost or renamed. Revision of the taxonomy of these extremely halophilic genera from Bacillus to Bacterium to Halobacterium reflected the increasing sophistication of our knowledge of the microbial world during this period, and these changes were generally accepted by the scientific community. In the modern era, there have been many more proposals for taxonomic revisions among these halophiles, some of which have been readily acceptable, and others that have since been challenged or refuted [5, 6].
A proposal to modernize haloarchaeal taxonomy and terminology
Among extremely halophilic microorganisms, the distinction of halophilic Archaea from halophilic Bacteria became apparent in the 1970's through the molecular phylogenetic work of Woese, who proposed the three-domain view of life. While halophilic microorganisms represented many different taxonomic groups in the bacterial domain, those in the archaeal domain fell into a single order (Halobacteriales) and family (Halobacteriacae) . Our understanding of the existence of the three domains has created ambiguity in the terminology used, since 'halobacteria' traditionally referred to all extremely halophilic microorganisms, including both halophilic Bacteria and halophilic Archaea. In order to clarify the definitions, we propose that the term halobacteria be reserved only for halophiles that are members of the bacterial domain, while haloarchaea be used only for halophiles that are members of the archaeal domain. In addition, on a taxonomic level, the order Halobacteriales should be designated as Haloarchaeales and the family Halobacteriaceae should be as Haloarchaeaceae. Finally, the Halobacterium genus would be better named Haloarchaeum to reflect its membership in the archaeal rather than the bacterial domain. These revisions would help update the taxonomy and terminology of halophilic microorganisms to be consistent with our current understanding of the microbial world.
Taxonomic ambiguity among species
While our proposed revision of haloarchaeal taxonomy is relatively simple, disentangling the taxonomy and pedigree within the original genus, Halobacterium (Haloarchaeum), is considerably more complex. Over the past twenty-five to fifty years, this genus witnessed a contraction in the number of recognized species from over a dozen to just a single species. While some acquired new genus designations (e.g. Halobacterium volcanii changed to Haloferax volcanii and Halobacterium marismortui to Haloarcula marismortui), in 1990, Grant and Larsen proposed combining three common species, Halobacterium halobium, H. salinarium, and H. cutirubrum, into a single one, H. salinarium . However, this proposal was not fully accepted by the community since the changes were not fully in accordance with the rules of the Bacteriological Code . In particular, halobium predated salinarium in the literature and, by convention, the former name should have taken precedence over the latter. To complicate matters further, in 1996, Ventosa and Oren proposed renaming of H. salinarium to H. salinarum , removing an "i", in their opinion, for linguistic reasons. However, many investigators dissented and continued to use the original species designations. In Euzéby's List of Prokaryotic Names with Standing in Nomenclature , he reported that salinarium, is derived from the Latin adjective salinarius a um, meaning "of salt works", while salinarum is derived from salinae arum, meaning "salt works", and concluded that salinarium was indeed correct. There is no doubt that naming and renaming of these species has left the taxonomy of Halobacterium (Haloarchaeum) species in disarray in the literature and in the haloarchaeal community.
Perhaps the worst case of taxonomic ambiguity is for the first Halobacterium (Haloarchaeum) isolate sequenced and also the most widely used haloarchaeal strain, which was published under the name H. halobium strain NRC-1 . The origin of strain NRC-1 is uncertain, though it likely appeared from the 1960's collection of W. Stoeckenius and was disseminated via W.F. Doolittle (personal communications) to S. DasSarma in the 1980's. In 2000, the NRC-1 strain was deposited by the DasSarma laboratory in the American Type Culture Collection (ATCC no. 700922) for standardization and distribution in the research community and has since been used by Carolina Biological Supply Company in the educational sphere (Carolina no. 154777) . Stocks of the original culture used for sequencing are also maintained in the DasSarma laboratory. As a result of uncertainties regarding the origin of this strain, the authors of the complete genome sequencing paper  dropped the species designation, reverting to "Halobacterium sp. strain NRC-1", while its pedigree was being rigorously established and the relationships within this group of organisms fully clarified. However, despite the lack of appearance of definitive information on the identity of NRC-1, Gruber et al.  published a paper in 2004 reclassifying the wild-type isolate as a strain of the 'H. salinarum' species. In so doing, these authors ignored a variety of differences between strains, including their own pulsed-field gel patterns, as well as variations in restriction maps of the unstable resident megaplasmids. Overreliance on phylogenetic trees based on 16S rRNA sequences, some of which are already known to be divergent even within single haloarchaeal species , was a serious shortcoming of this study.
Reexamination of the available data on Halobacterium (Haloarchaeum) isolates at the phylogenetic and taxonomic levels confirms the existence of serious complications. The 16S rRNA sequences vary in nearly all of the originally distinct species, with that of NRC-1 and H. salinarium differing in several positions. Differences also exist between the NRC-1 and H. halobium 23S rRNA sequences, as well as between NRC-1 and H. cutirubrum 5S rRNA. In fact, a recent publication even reported a major deletion in the 16S rRNA promoter region of 'H. salinarum' (originally H. halobium) strain R-1 in comparison to strain NRC-1 and other similar strains . The most compelling case for the existence of substantial taxonomic diversity among Halobacterium (Haloarchaeum) isolates is from the recent genotyping analysis of Cleland et al. using the DiversiLab repPCR system . In this study, some of the seven Halobacterium (Haloarchaeum) strains in the ATCC collection show differences quantitatively similar to haloarchaea that are classified as different genera of the Halobacteriaceae (Haloarchaeaceae) family. Their examination of NRC-1 by this method (Figure 1) showed that this strain fell below 70% similarity compared to other 'H. salinarium' strains in ATCC, suggesting that NRC-1 should be given an entirely new species designation using that criterion. This analysis supports our viewpoint that it is premature to reclassify all of these Halobacterium (Haloarchaeum) isolates as a single species, especially without an existing consensus in the community on the definition of what constitutes a "species" among these organisms.
Phenotypes and pedigree
At the phenotypic level, Halobacterium (Haloarchaeum) strains have some clear-cut differences. An especially striking difference is the absence, in 'H. salinarum' (e.g. strain R-1), of gas vesicles, which are characteristic of Halobacterium sp. NRC-1. Gas vesicles permit NRC-1 to move vertically in the water column in response to oxygen, light, and temperature, and the corresponding expression of gas vesicle protein genes can be clearly seen in DNA microarray experiments [17, 18]. The lack of gas vesicles in 'H. salinarum' indicates that these organisms exist in significantly different environments from NRC-1, with the latter inhabiting dynamic ones, and the former in more constant environments. This notable phenotypic difference is easily visible even to the naked eye. Some other Halobacterium (Haloarchaeum) species contain two different morphological types of gas vesicles in the same cell, those which are narrower and diagnostic of species inhabiting deep habitats, as well as those which are wider and found in shallow brines (like NRC-1), suggesting the widespread environmental distribution of these species. Examined more broadly, isolate-specific differences have been shown to exist at the level of antibiotic-resistance markers, measured cations in cells, and protein, lipid, and sugar content in the cell envelopes .
The pedigree of the various strains of Halobacterium (Haloarchaeum) being studied in laboratories worldwide is also confounding. One example is the unclear relationship between NRC-1 and R-1, and another similar strain S-9, a purple membrane overproducer. While some investigators reported that NRC-1, R-1, and S-9, were very close relatives , others indicated otherwise [20, 21]. The stable gas vesicle-deficient strain, R-1, may be the parent of S-9, a strain which was isolated after extensive chemical mutagenesis, but R-1 is probably not a descendent of NRC-1. Not surprisingly, the genome sequencing results showed at least 200 kb of additional DNA in R-1 compared to NRC-1 and very little similarity in their resident megaplasmids . Many additional examples of incongruent taxonomy and pedigree among Halobacterium (Haloarchaeum) species are reviewed by Grant and Larsen  and Tindall . Clearly, the frequent and questionable revisions in the taxonomy of these interesting microbes, and at times, the lack of careful maintenance and documentation of their pedigree, are a serious impediment to advancing the field.
The incredible precision of the genomic era has empowered microbiologists with the genetic blueprints of more than a thousand microorganisms and allowed for the development of many new approaches for the interrogation of their biology. Unfortunately, studies of certain microbes, such as the haloarchaea, have been made exceedingly difficult by the arbitrary and unnecessary renaming of strains, poor record keeping of pedigree, and the lack of a universal definition of species. All of these shortcomings make it likely that future generations will not be able to fully interpret and utilize the current literature, ultimately diminishing the contributions of both past and present generations. While rigorous genetic studies and complete genome sequences are destined to make a permanent contribution to the field, taxonomic rearrangements based on inadequate experimentation and flawed logic hold it back. Although these points resonate especially true among the haloarchaea, we suspect that similar taxonomic issues and challenges are quite widespread among other prokaryotes as well and are deserving of further scrutiny.
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We thank Dr. David Cleland for Figure 1 and helpful discussions and Professor Martin Bloomer for his expertise with Greek and Latin language issues.
The authors declare that they have no competing interests.
Both authors contributed to the development of the concepts and writing of the manuscript.
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DasSarma, P., DasSarma, S. On the origin of prokaryotic "species": the taxonomy of halophilic Archaea. Aquat. Biosyst. 4, 5 (2008). https://doi.org/10.1186/1746-1448-4-5
- Halophilic Bacterium
- Purple Membrane
- Microbial World
- Halophilic Microorganism
- Bacterial Domain