Figure 7
From: Heterologous ectoine production in Escherichia coli: By-passing the metabolic bottle-neck
Construction of the plasmids pAKECT1. Plasmid pAKECT1 (10.1 kb) was constructed from pOSM12 and pRK1. Only donor plasmids, final construct and the relevant restriction sites are shown. Due to lack of suitable restriction sites, a complex construction scheme had to be applied (details in text). ectUp: region upstream of ectA with putative osmoregulated promoter sequences. lysC: deregulated aspartate kinase from Corynebacterium glutamicum MH20-22B.