Table 1 Phylogenetic affiliations of the uncultured bacteria based on 16S rDNA analysis
From: Molecular identification of microorganisms associated with the brine shrimp Artemia franciscana
Sequence | Clones | Closest BLASTn match to NCBI nr database | Identities (%) | Genus [confidence value] | Genbank Accession |
|---|---|---|---|---|---|
A. Sequences obtained from GSL Adult Artemia | |||||
ABC1 | 9 | DQ068937.1 Vibrio metschnikovii | 627/631 (99) | Vibrio[100%] | KC696895-KC696903 |
ABC2 | 5 | AB362696.1 Halolactibacillus halophilus | 615/631 (97) | Halolactibacillus[82%] | KC696904-KC696906 |
ABC3 | 15 | DQ351910.1 Uncultured bacterium clone JH-WH17 | 592/603 (98) | Halomonas[100%] | KC696872-KC696886 |
ABC4 | 4 | AM691100.1 Uncultured “Rhodobacteraceae” | 582/589 (98) | Roseovarius[100%] | KC696887-KC696892 |
ABU1 | 1 | EU245085.1 Uncultured organism clone MAT-CR-H1-G02 | 629/661 (95) | Alkalilimnicola[16%] | KC696869 |
ABU4 | 1 | AM420114.1 Uncultured alpha proteobacterium | 532/532 (100) | Caulobacter[98%] | KC696870 |
ABU6 | 1 | DQ396185.1 Uncultured organism clone ctg_NISAA81 | 581/587 (98) | Halomonas[100%] | KC696871 |
ABU7 | 1 | DQ154838.1 Uncultured bacterium clone GN01-0.012 | 513/534 (96) | Lutibacter [15%] | KC696893-KC696894 |
B. Sequences obtained from GSL Artemia cysts | |||||
GBRC1 | 14 | DQ462298.1 Uncultured bacterium clone e41 | 525/531 (98) | Idiomarina[100%] | KC703296-KC703308 |
GBRC2 | 4 | X95527.1 SCRR701T S. costicola (strain NCIMB 701-T) | 496/497 (99) | Salinivibrio[100%] | KC70329-KC703295 |
GBRU1 | 1 | EU287134.1 Uncultured bacterium clone P13-41 | 406/430 (94) | Marinimicrobium[96%] | KC703293 |
GBRU2 | 1 | EF190068.1 Uncultured Psychroflexus sp. clone GSX1 | 543/587 (92) | Gramella[41%] | KC703291 |
GBRU3 | 1 | DQ157009.1 “Marinobacter haloterrigenus” strain FP2.5 | 488/518 (94) | Marinobacter[54%] | KC703292 |
C. Sequences obtained from SFB strain Artemia cysts | |||||
SBR1 | 15 | X95527.1 SCRR701T S. costicola (strain NCIMB 701-T) | 618/622 (99) | Salinivibrio[100%] | KC696910-KC696930 |
SBR2 | 3 | EF177666.1 Idiomarina sp. Y24 | 564/565 (99) | Idiomarina[100%] | KC696931-KC696933 |
SBU1 | 1 | EU135665.1 Lysobacter sp. YIM C734 | 566/596 (94) | Lysobacter[75%] | KC696909 |
SBU2 | 1 | AF505746.1 Gamma proteobacterium UMB21A | 562/566 (99) | Psychrobacter[100%] | KC696907 |
SBU3 | 1 | AM084035.1 Acidovorax sp. R-25076 | 564/564 (100) | Acidovorax[100%] | KC696908 |
D. Sequences obtained from GSL water | |||||
HBRC2 | 4 | AM691089.1 Uncultured Psychroflexus sp. isolate EG26 | 554/559 (99) | Psychroflexus[100%] | KC696938-KC696940, KC696944-KC696945 |
HBRC3 | 4 | AM691089.1 Uncultured Psychroflexus sp. isolate EG26 | 531/546 (97) | Psychroflexus[99%] | KC696935-KC696937, KC696941-KC696943 |
HBRC4 | 9 | EF554887.1 Halomonas sp. G27 | 512/527 (97) | Halomonas[100%] | KC696946-KC696952, KC696963-KC696966 |
HBRC5 | 7 | AY862776.2 Uncultured proteobacterium clone At18AugB10 | 492/492 (100) | Alkalilimnicola[74%] | KC696953-KC696962 |
HBUN1 | 1 | AY862797.2 Uncultured actinobacterium clone At12OctB10 | 413/418 (98) | Agrococcus [59%] | KC696934 |