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Table 2 Microorganisms present in Tirez lagoon water column and sediments identified by microscopic observation, clone-sequencing, DGGE band-sequencing and sequencing of culture isolates

From: Microbial community composition of Tirez lagoon (Spain), a highly sulfated athalassohaline environment

ID (Access no.) a Closest relative b No. of occurrences c
   Rw Dw Rs Ds
Bacteria      
Actinobacteria      
Rw_cb_7 (FJ172065) Uncultured actinobacterium clone TDNP_LSbc97_3_28_94 (FJ516859). Semiarid wetland (Central Spain) [97%] 1    
Microbacteriaceae [100%], Cryobacterium [57%]
Rw_db_11 (EU722702) Uncultured organism clone SBYG_2070 (JN450257). Guerrero Negro hypersaline microbial mat [99%] 1    
Actinomycetales [100%], Microbacteriaceae [97%], Schumannella [28%]
Rs_ib_2A (EU722709) Arthrobacter phenanthrenivorans strain Sphe3 (NR_074770) [97%]    2  
Micrococcaceae [100%], Arthrobacter [49%]
Bacteroidetes      
Rw_cb_8 (EU725593) Uncultured marine bacterium clone BM1-F-27 (FJ826125). Yellow Sea [97%] 1    
Flavobacteriales [100%], Flavobacteriaceae [97%]
Ds_db_31 (EU722701) Uncultured bacterium clone BJGMM-1s-364 (JQ800813). Soil samples from the Yellow River DeltaYellow River [98%]     1
Flavobacteriaceae [100%], Gillisia [99%]
Rs_cb_74 (EU722650) Uncultured bacterium clone a43 (HM468007). Wastewater treated with with ferrous salt [99%]    1  
Sphingobacteriales [100%], Cyclobacteriaceae [81%], Nitritalea [81%]
Ds_db_28 (EU722700) Muricauda flavescens strain SW-62 (NR_042908). Salt lake, Korea [82%]     1
Bacteria [100%], Bacteroidetes [87%]
Rs_cb_66 (EU722655) Uncultured organism clone MAT-CR-P2-E05 (EU246052). Hypersaline microbial mat [96%]    1  
Marinilabiaceae [100%], Anaerophaga [99%]
Rs_db_2 (FJ172074) Uncultured bacterium clone 184-32 (GU212609). Saline soil samples Qaidam Basim, China [97%]    1  
Marinilabiaceae [100%], Anaerophaga [66%]
Flavobacteria      
Rs_db_12 (EU722690) Uncultured Bacteroidetes bacterium clone CF07-19 (FJ844036). High mountain lake, China [93%]     1
Flavobacteriaceae [100%], Psychroflexus [55%]
Rw_cb_19 (EU725598) Psychroflexus sediminis strain YIM C238 (NR_044410). Haloalkaline soil [98%] 1    
Flavobacteriaceae [100%], Psychroflexus [100%]
Rs_cb_27 (EU722652) Psychroflexus sediminis strain YIM C238 (NR_044410). Haloalkaline soil [98%]    2  
Flavobacteriaceae [100%], Psychroflexus [100%]
Rs_db_7 (EU722686) Uncultured Salegentibacter sp. clone HAHS13.025 (HQ397000). Haloalkaline soil [98%] 2   5  
Flavobacteriaceae [100%], Salinimicrobium [95%]
Rw_cb_17 (EU725597) Psychroflexus sediminis strain YIM C238 (NR_044410). Haloalkaline soil [98%] 1    
Flavobacteriaceae [100%], Psychroflexus [100%]
Rs_cb_80 (EU722656) Uncultured bacterium clone MBFOS-06 (EU369165). Oyster shell [95%]    1  
Flavobacteriaceae [100%], Salinimicrobium [99%]
Sphingobacteria      
Ds_db_20 (EU722696) Sphingobacteria bacterium clone A1503 (EU283512). Anderson lake [95%]     1
Bacteroidetes [100%], Flavobacteria [50%]
Bacilli      
Rs_db_13 (EU722691) Uncultured bacterium clone H0014 (JX391054). Marine sediment [90%]    2 1
Firmicutes [99%], Bacilli [93%], Bacillales [81%]
Rs_db_4 (FJ172072) Uncultured bacterium clone B-2 (HQ703872). Qinghai lake sediment [98%]    1  
Bacteria [100%], Firmicutes [92%], Bacilli [65%]
Ds_db_22 (EU722698) Uncultured bacterium clone Kasin-B2-B05 (HE604654). Hypersaline sediments [99%]    1  
Sporolactobacillaceae [100%], Sporolactobacillaceae incertae sedis [100%]
Ds_ib_4 (EU722711) Bacillus sp. DHC09 (JQ904720). Sea surface sediment [97%]     1
Bacillales [100%], Bacillaceae 1 [80%], Falsibacillus [71%]
Rs_ib_3A (FJ172083) Paenibacillus sp. 5-3 (HQ832503) Food waste [92%]    2  
Bacilli [97%], Bacillales [97%], Paenibacillaceae 1 [80%]
Rs_ib_4iA (FJ172086) Paenibacillus sp. ITCr59 (FR823415). Agricultural soil [97%]    5  
Paenibacillaceae 1 [100%], Fontibacillus [95%]
Clostridia      
Rs_db_29 (FJ172073) Uncultured bacterium clone H3034 (JX391174). Marine sediment [98%]     1
Clostridiales [100%], Clostridiaceae 3 [99%], Sporosalibacterium [96%]
Alphaproteobacteria      
Rw_cb_4 (EU725591) Uncultured Rhodobacteraceae clone DS127 (DQ234210). River estuary [98%] 1    
Rhodobacteraceae [100%], Roseovarius [86%]
Rw_cb_6 (FJ172064) Roseobacter sp. B11 (DQ659411) [99%] 2    
Rhodobacteraceae [100%], Seohaeicola [98%]
Rw_cb_2 (FJ172062) Loktanella vestfoldensis strain R-9477 (NR_029021) [98%] 12    
Rhodobacteraceae [100%], Loktanella [100%]
Gammaproteobacteria      
Rs_cb_73 (EU722649) Uncultured Idiomarina sp. clone XJ120 (EF648161). Aerobic activated sludge [94%]    1  
Idiomarinaceae [100%], Pseudidiomarina [99%]
Rs_cb_1 (FJ236711) Pseudidiomarina sp. YCSA4 (GQ246209). Water of a salt field [93%]    3  
Idiomarinaceae [100%], Pseudidiomarina [100%]
Ds_ib_6 (FJ172080) Pseudidiomarina homiensis strain CT34 (HM854277) [99%]     4
Idiomarinaceae [100%], Idiomarina [81%]
Rs_cb_93 (EU722651) Uncultured proteobacterium clone TY4SP11r (JQ218797). Marine macro-alga [95%]    1  
Idiomarinaceae [100%], Pseudidiomarina [98%]
Rs_cb_26 (EU722643) Pseudidiomarina sp. 2PR54-15 (EU440967) [96%]    9  
Idiomarinaceae [100%], Idiomarina [92%]
Ds_ib_11 (EU722714) Uncultured gamma proteobacterium clone XJ85 (EF648142). Aerobic activated sludge [98%]     1
Idiomarinaceae [100%], Pseudidiomarina [59%]
Ds_ib_3 (EU722710) Marinobacter adhaerens strain S20-1 (KC420687) [100%]    1 1
Alteromonadaceae [100%], Marinobacter [100%]
Rs_db_16 (EU722694) Uncultured bacterium clone SN18 (JQ824910). Saline and alkaline soil [97%]    1  
Gammaproteobacteria [99%], Alteromonadales [52%], Alteromonadaceae [52%]
Rw_cb_9 (FJ172066) Uncultured bacterium clone SN26 (JQ824918). Saline and alkaline soil [98%] 1   1  
Piscirickettsiaceae [100%], Methylophaga [100%]
Rw_cb_46 (FJ172063) Uncultured bacterium clone SINP962 (HM127832). Qinghai lake [91%] 1    
Bacteria [100%], Proteobacteria [61%], Gammaproteobacteria [61%]
Rs_cb_94 (FJ236710) Uncultured bacterium clone Lupin-1130m-2-pse1 (EF200114). Subpermafrost fracture waters in Artic [94%]    1  
Proteobacteria [99%], Gammaproteobacteria [98%], Oceanospirillales [47%]
Dw_ib_7 (EU722712) Pseudoalteromonas sp. TA010_3 (EU308473). Solar saltern [98%]   1   
Pseudoalteromonadaceae [100%], Pseudoalteromonas [100%]
Rs_db_10 (EU722688) Halomonas sp. HL33 (KC705271). Hot lake hypersaline margin soil [95%]    3 1
Halomonadaceae [100%], Cobetia [41%]
Ds_ib_8 (EU722713) Uncultured Halomonas sp. clone BPS_CK65 (HQ857613). Hydrocarbon contaminated saline [98%]     9
Halomonadaceae [100%], Halomonas [98%]
Rs_cb_64 (FJ172071) Halomonas sediminis strain YIM C248 (EU135707) [98%]    1  
Halomonadaceae [100%], Halomonas [83%]
Rs_db_15 (EU722693) Uncultured bacterium clone 100307_0m_01F (KC358335). Low salinity soda lake [95%]    1  
Piscirickettsiaceae [100%], Thioalkalimicrobium [94%]
Deltaproteobacteria      
Rs_db_srb (EU722708) Uncultured bacterium clone E6bG07 (DQ103666). Hypersaline endoevaporitic microbial mat [96%]    1  
Desulfohalobiaceae [100%], Desulfohalobium [82%]
Cyanobacteria      
  Nostoc sp. (*) *    
  Anabaena sp. (*) *    
  Pseudoanabaena sp. (*) *    
  Nodularia sp. (*) *    
Rw_ib_C (FJ172091) Leptolyngbya sp. LEGE 07084 (HM217072). Temperate estuary [98%] 2    
Cyanobacteria [100%], Family IV [100%], GpIV [100%]
Rw_db_6 (FJ172075) Uncultured organism clone SBXY_5108 (JN429822). Hypersaline microbial mat [99%] 1    
Cyanobacteria [100%], Family XIII [78%], GpXIII [78%]
Archaea      
Halobacteria      
Rs_ca_8 (EU722666) Uncultured haloarchaeon clone XKL10 (JN714413). Saline lake [99%]    1  
Halobacteriaceae [100%], Halobacterium [46%]
Rs_ca_62 (EU722680) Uncultured haloarchaeon clone XKL44 (JN714440). Saline lake [98%]    3  
Halobacteriaceae [100%], Halolamina [100%]
Rs_ca_16 (EU722670) Uncultured haloarchaeon clone XKL11 (JN714414). Saline lake [96%]    1  
Halobacteriaceae [100%], Halococcus [99%]
Rs_ca_29 (EU722669) Uncultured haloarchaeon clone XKL23 (JN714423). Saline lake [96%]    1  
Halobacteriaceae [100%], Halomicrobium [88%]
Rs_ca_64 (EU722681) Halobacteriaceae archaeon EA3 (HQ197981). Salt lake brine [99%]    1  
Halobacteriaceae [100%], Halolamina [100%]
Rs_ca_10 (EU722677) Halobacteriaceae archaeon R30 (HM159607). Salted kelp [97%]    1  
Halobacteriaceae [100%], Halonotius [97%]
Rs_ca_7 (EU722667) Uncultured Halobacterium sp. clone 7A23 (AY987826). Maras salterns [99%]    2  
Halobacteriaceae [100%], Halobacterium [100%]
Dw_ca_51 (FJ172059) Halobacterium sp. AUS-2 (D32082) [97%]   2   
Halobacteriaceae [100%], Halorubrum [100%]
Rs_ca_5 (EU722663) Uncultured euryarchaeote clone DSFBPENV12arc_7C (KC465576). Brine pool water [99%]    7  
Halobacteriaceae [100%], Halobacterium [66%]
Ds_da_7 (FJ172052) Uncultured archaeon clone Kasin-A3-B06 (HE604580). Exposed salt lake sediment [99%]     1
Halobacteriaceae [100%], Salarchaeum [56%]
Rs_ca_49 (EU722671) Uncultured haloarchaeon clone TX4CA_35 (EF690590). Alkaline-saline soil [94%]    1  
Halobacteriaceae [100%], Halococcus [69%]
Dw_ca_34 (FJ172057) Halorubrum kocurii (AB576124) [99%]   4 2  
Halobacteriaceae [100%], Halorubrum [100%]
Dw_ca_36 (FJ172058) Halorubrum xinjiangense strain BD-1 (NR_028205) [99%]   1   
Halobacteriaceae [100%], Halorubrum [100%]
Dw_ca_59 (FJ172060) Uncultured euryarchaeote clone SFH1E051 (FN391283). Solar saltern sediment [99%]   1   
Halobacteriaceae [100%], Halorhabdus [43%]
Rs_ca_31 (EU722657) Uncultured haloarchaeon clone XKL48 (JN714443). Saline lake [99%]    1  
Halobacteriaceae [100%], Natronomonas [100%]
Rs_ca_59 (EU722658) Uncultured haloarchaeon clone XKL48 (JN714443). Saline lake [99%]    1  
Halobacteriaceae [100%], Natronomonas [100%]
Ds_da_3 (EU722706) Natronobacterium sp. isolate 2-24-8 (AJ878084) [97%]     1
Halobacteriaceae [100%], Natronomonas [100%]
Rs_ca_93 (EU722674) Halomicrobium katesii (JN120802) [99%]    1  
Halobacteriaceae [100%], Halomicrobium [100%]
Rs_ca_52 (EU722672) Haloarcula sp. AB19 (DQ471854) [99%]    1  
Halobacteriaceae [100%], Haloarcula [100%]
Rs_ca_21 (EU722673) Halophilic archaeon strain BNERC31 (AB766180). Solar saltern [96%]    1  
Halobacteriaceae [100%], Haloarcula [100%]
Archaeoglobi      
Rs_da_2 (EU722705) Uncultured archaeon clone 11 (GQ452803). Hypersaline methane seep in canadian high Artic [97%]    2  
Archaeoglobales [98%], Archaeoglobaceae [98%], Archaeoglobus [63%]
Candidate division MSBL1      
Rs_ca_41 (EU722682) Uncultured euryarchaeote clone Discovery_a (HQ530525). Hydrothermal brine system in the Red Sea [93%]    1  
Euryarchaeota [96%], Archaeoglobi [49%], Archaeoglobales [49%]
Eukarya      
Bascilariophyceae      
Rw_ie_diat (FJ172078) Nitzschia communis strain FDCC L408 (AJ867278) [99%] 1    
Monogononta      
Hexarthra sp. (*)   *    
Branchiopoda      
Artemia sp. (*)   *    
Chlorophyceae      
Dunaliella sp. (*)   * *   
Dinophyceae      
Rw_ie_din (EU734574) Woloszynskia cincta strain MALINA FT56.6 PG8 (JN934667) [98%] 1    
Eurotiomycetes      
Aspergillus sp. (*)      *
  1. a) The suffixes define technique of identification and Domain: clone library of Bacteria (cb), DGGE gel band of Bacteria (db), isolate of Bacteria (ib), clone library of Archaea (ca), DGGE gel band of Archaea (da) and microscopic identification (*).
  2. b) The sequence identity (%) was determined by two methods: BLASTN in the GenBank database (first line of the cell) [25] and Classifier in the RDP (second line of the cell) [26].
  3. c) The prefixes define the origin of the samples: (Dw) Water from dry season, (Rw) Water from rainy season, (Rs) Sediment from rainy season, (Ds) Sediment from dry season.