TY - JOUR AU - Bozo-Hurtado, Lorelei AU - García-Amado, M. Alexandra AU - Chistoserdov, Andrei AU - Varela, Ramon AU - Narvaez, J. Jesus AU - Colwell, Rita AU - Suárez, Paula PY - 2013 DA - 2013/08/28 TI - Identification of bacteria in enrichment cultures of sulfate reducers in the Cariaco Basin water column employing Denaturing Gradient Gel Electrophoresis of 16S ribosomal RNA gene fragments JO - Aquatic Biosystems SP - 17 VL - 9 IS - 1 AB - The Cariaco Basin is characterized by pronounced and predictable vertical layering of microbial communities dominated by reduced sulfur species at and below the redox transition zone. Marine water samples were collected in May, 2005 and 2006, at the sampling stations A (10°30′ N, 64°40′ W), B (10°40′ N, 64°45′ W) and D (10°43’N, 64°32’W) from different depths, including surface, redox interface, and anoxic zones. In order to enrich for sulfate reducing bacteria (SRB), water samples were inoculated into anaerobic media amended with lactate or acetate as carbon source. To analyze the composition of enrichment cultures, we performed DNA extraction, PCR-DGGE, and sequencing of selected bands. SN - 2046-9063 UR - https://doi.org/10.1186/2046-9063-9-17 DO - 10.1186/2046-9063-9-17 ID - Bozo-Hurtado2013 ER -