Variation in spatial and temporal incidence of the crustacean pathogen Hematodinium perezi in environmental samples from Atlantic Coastal Bays

Table 2 Levels of H. perezi from Indian River Inlet samples

Indian River Environmental samples
	Sampling date	Positive sites	Sediment type	Gene copies/g sediment
Sediment	07.01.08	IR7	sandy clay	1.0E + 04
	07.29.08	IR2-1	silty sand	5.9E + 03
		IR2-2	sand	4.4E + 03
		IR3	silty sand	5.8E + 03
	07.15.09	IR1a	silty sand	2.0E + 02
		IR2-1 c	silty sand	1.5E + 02
		IR3a	silt	1.7E + 03
		IR3b	silt	9.5E + 02
		IR3c	silty sand	2.3E + 02
		IR4a	silty sand	1.7E + 02
		IR4b	silty sand	1.1E + 03
		IR4c	silty sand	3.6E + 02
		IR7a	sand	1.5E + 03
		IR8c	silt	2.2E + 02
		IR10b	silt	2.3E + 02
		IR12b	silt	4.0E + 02
				Gene copies/L water
Water	07.15.09	IR1	n/a	6.1E + 02
		IR3	n/a	4.0E + 02
		IR11	n/a	1.3E + 02
		IR12	n/a	8.2E + 01

Quantitative PCR was used to assess H. perezi in DNA extracted from environmental samples from 13 locations in Indian River Inlet, DE (Figure 3) Samples were collected at indicated dates in July 2008 and 2009. In July 2009, each site was sampled in triplicate, as indicated by suffixes a, b, and c. Results are reported as H. perezi ribosomal gene copy number per g sediment and gene copies per L of water. Note that 12.09.08 sediment samples were collected but were all negative.