Schematic representation of H. perezi rDNA locus. Figure shows locations of PCR primer pairs (Table 1), relative to SSU, ITS, 5.8S, and LSU genes as defined in GenBank entry JQ815886. PlaceF/R primers are from Nagle et al., and target the SSU gene. Primers Schott F/R are from Pitula et al.  and amplify the 5.8S gene. Primers ITS2F/R were developed in the current study. The star and diamonds indicate locations of indel and single nucleotide polymorphisms, respectively, between clones of H. perezi derived from independent infections of C. sapidus. Polymorphisms are annotated in GenBank JQ815886.