Analysis of PRL mRNA level in cinnamon clownfish A. melanopus upon exposure to 15 ppt. Total RNA(0.5μg) prepared from pituitary gland was used for generating cDNA by reverse transcription followed by PCR amplification using PRL-specific primers. Levels of β-actin mRNA were evaluated as a control. The expression level of each prolactin mRNA was normalized with respect to the level of β-actin transcript. Each value represents the mean±SE (n=4) and the same letters indicate no significant difference (P>0.05).