Cell growth and PHB production by H. boliviensis using different combinations of carbohydrates and molasses. (B) PHB accumulation in batch culture using 1.5% (w/v) sucrose and 1% (w/v) glucose. Assay numbers refer to the concentration of carbohydrates added to the medium, %(w/v): 1) 2.5 sucrose, 2) 2.0 sucrose and 0.5 glucose, 3) 1.5 sucrose and 1 glucose, 4) 1.0 sucrose and 1.5 glucose, 5) 0.3 sucrose, 0.7 glucose and 1.5 dried molasses and 6) 2.5 dried molasses. All experiments were performed in shake flasks at 35°C and 220 rpm of agitation. All experiments were performed in triplicate. The error bars refer to the SD of the average values.