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Table 1 Salinity, EcoPlate™ utilization, and isolate characteristics for samples taken from La Sal del Rey 2010.

From: Phenotypic characterization and 16S rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, La Sal del Rey, in extreme South Texas (USA)

Sample Salinity (ppt) Preferred
EcoPlate™ Substrate*
Isolates
    N Gram-negative Rod-shaped Percent catalase positive
A 4 L-phenylalanine 20 15 14 100
B 56 Glycogen 20 16 16 95
C 420 Itaconic Acid 10 2 9 100
D 4 Tween 40 17 14 4 100
E 37 L-arginine 17 15 12 100
F 280 D-cellobiose 16 14 16 100
G 86 D-malic acid nd nd nd nd
H 220 Hydroxybutyric acid nd nd nd nd
  1. * Highest positive EcoPlate™ absorbance value
  2. One isolate from site B became contaminated and was eliminated from the study.
  3. nd = not determined
  4. Samples A - F were taken on 14 June; samples G - H were taken on 5 July after Hurricane Alex made landfall. Salinity was measured using a handheld refractometer. EcoPlates™ were inoculated with water or diluted soil samples, incubated at 25°C, and measured with a spectrophotometer (595 nm) at 24 h, 48 h and 72 h. EcoPlates™ results shown are the final results at 72 h. Isolates were selected from amongst all the samples. Some isolates contained a mixture of cell types.