Calcium and zinc concentrations were determined in media and membrane associated by ICP-OES. Halomonas halophila were cultivated in MH 4 and MH 4Z medium. Samples at 1, 3, 7, 9, and 11 days were analyzed. Black columns: MH 4Z medium, white columns: MH 4 medium. Concentrations of calcium and zinc are indicated in the columns in μg/mL. The concentration of membrane associated calcium at day 1 was not determined (n.d.) due to cell density limitations.