Table 1 Geographical origin and gene sequence accession details of Dunaliella strains studied in the present work
Groups | Strain code | Isolated from | Geographic co-ordinates | Month of collection | Salinity of the sampled water | 18S rDNA product size | Genebank accession No. | ||
|---|---|---|---|---|---|---|---|---|---|
18S rDNA | ITS region | rbcL gene | |||||||
| Â | CS265 | Dunaliella salina; Reference strain from CSIRO collection of living microalgae, Australia | 2210 bp | JN807321 | JN797804 | JN797820 | |||
I | MBTD-CMFRI-S135 | Sea water, Calicut, Kerala (WC) | 11°15’ N 75°46’ E | May 2009 | 33 ppt | 2230 bp | JF708161 | JN797802 | JN797818 |
MBTD-CMFRI-S089 | Kelambakkom salt pan, Chennai, TN (EC) | Culture maintained in CMFRI phytoplankton culture collection, isolated from Chennai salt pan. | 2210 bp | JF708173 | JN797806 | JN797811 | |||
II | MBTD-CMFRI-S118 | Salt pan, Nellore, AP (EC) | 14°16’ N 80°07’ E | March 2009 | 300 ppt | 2290 bp | JN807316 | JN797808 | JN797813 |
MBTD-CMFRI-S086 | Salt pan, Tuticorin, TN, (EC) | 08°47’ N 78°09’ E | February 2009 | 300 ppt | 2290 bp | JF708169 | JN797805 | JN797810 | |
MBTD-CMFRI-S121 | Pulicat salt lake, AP (EC) | 13°40’ N 80°11’ E | March 2009 | 150 ppt | 2250 bp | JN807317 | JN797809 | JN797814 | |
III | MBTD-CMFRI-S115 | Kelambakkom salt pan, Chennai, TN (EC) | 12°45’ N 80°12’ E | March 2009 | 380 ppt | 2550 bp | JN807315 | JN797807 | JN797812 |
MBTD-CMFRI-S122 | Salt pan, Ribandar, Goa (WC) | 15°30’ N 73°51’ E | May 2009 | 280 ppt | 2550 bp | JN807318 | JN797799 | JN797815 | |
MBTD-CMFRI-S133 | Salt pan, Kutch, Gujarat (WC) | 23°50’ N 69°39’ E | July 2009 | 320 ppt | 2530 bp | JF708183 | JN797801 | JN797817 | |
IV | MBTD-CMFRI-S125 | Salt pan, Pilar, Goa (WC) | 15°26’ N 73°53’ E | May 2009 | 260 ppt | 2640 bp | JN807319 | JN797800 | JN797816 |
V | MBTD-CMFRI-S147 | Salt pan, Kutch, Gujarat (WC) | 23°50’ N 69°39’ E | April 2009 | 180 ppt | 1820 bp | JN807320 | JN797803 | JN797819 |