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Table 1 Geographical origin and gene sequence accession details of Dunaliella strains studied in the present work

From: Phenotypic and genetic characterization of Dunaliella (Chlorophyta) from Indian salinas and their diversity

Groups Strain code Isolated from Geographic co-ordinates Month of collection Salinity of the sampled water 18S rDNA product size Genebank accession No.
18S rDNA ITS region rbcL gene
  CS265 Dunaliella salina; Reference strain from CSIRO collection of living microalgae, Australia 2210 bp JN807321 JN797804 JN797820
I MBTD-CMFRI-S135 Sea water, Calicut, Kerala (WC) 11°15’ N 75°46’ E May 2009 33 ppt 2230 bp JF708161 JN797802 JN797818
MBTD-CMFRI-S089 Kelambakkom salt pan, Chennai, TN (EC) Culture maintained in CMFRI phytoplankton culture collection, isolated from Chennai salt pan. 2210 bp JF708173 JN797806 JN797811
II MBTD-CMFRI-S118 Salt pan, Nellore, AP (EC) 14°16’ N 80°07’ E March 2009 300 ppt 2290 bp JN807316 JN797808 JN797813
MBTD-CMFRI-S086 Salt pan, Tuticorin, TN, (EC) 08°47’ N 78°09’ E February 2009 300 ppt 2290 bp JF708169 JN797805 JN797810
MBTD-CMFRI-S121 Pulicat salt lake, AP (EC) 13°40’ N 80°11’ E March 2009 150 ppt 2250 bp JN807317 JN797809 JN797814
III MBTD-CMFRI-S115 Kelambakkom salt pan, Chennai, TN (EC) 12°45’ N 80°12’ E March 2009 380 ppt 2550 bp JN807315 JN797807 JN797812
MBTD-CMFRI-S122 Salt pan, Ribandar, Goa (WC) 15°30’ N 73°51’ E May 2009 280 ppt 2550 bp JN807318 JN797799 JN797815
MBTD-CMFRI-S133 Salt pan, Kutch, Gujarat (WC) 23°50’ N 69°39’ E July 2009 320 ppt 2530 bp JF708183 JN797801 JN797817
IV MBTD-CMFRI-S125 Salt pan, Pilar, Goa (WC) 15°26’ N 73°53’ E May 2009 260 ppt 2640 bp JN807319 JN797800 JN797816
V MBTD-CMFRI-S147 Salt pan, Kutch, Gujarat (WC) 23°50’ N 69°39’ E April 2009 180 ppt 1820 bp JN807320 JN797803 JN797819
  1. NB: For convenience strain codes used in text included only third part of full strain code (e.g., S086). AP, Andhra Pradesh; TN, Tamil Nadu; WC-west coast; EC, east coast.
  2. Indian strains were grouped into subsets based on the 18S rDNA size obtained by PCR amplification with MA1-MA2 primers.