Table 1 Primer sequences for cloning of the full-length cDNA of C. sapidus aquaporin-1 ( CasAQP-1 ) and qRT-PCR assays
|  | Primer sequence (5′ to 3′) |
|---|---|
CasAQPdF1 | GGNCAYATHWSKGGHGSHCA |
CasAQPdF2 | CAYATHAAYCCNGCNGTNAC |
CasAQPdR1 | GGNCCNAYCCARWANAYCCA |
CasAQPdR2 | AAMSWNCKRGCDGGRTTCAT |
CasAQP-1-3F1 | CTGGTCGCCCGCTACGTGT |
CasAQP-1-3F2 | CCCTGCTCTACATCATGGCCCAGTG |
CasAQP-1-5R1 | CCTGTCCCTTTGACATCGTT |
CasAQP-1-5R2 | CCGAACACCGTCAGCACGAGAATGA |
CasAQP-1-QF | CTCACGCCACAGGAAAAGCAAG |
CasAQP-1-QR | CAGCACGAGAATGAAGGTAATGAGG |