Figure 1

A) The nucleotide and deduced amino acid sequences of cDNA encoding CasAQP-1 (GenBank JQ970426) obtained from the hypodermis of C. sapidus . The start codon (ATG) and stop codon (TAA) are in bold, and underlined and marked with a ‘*’, respectively. The first 94 nt and the last 63 nt are italicized for the 5′ and 3′ UTRs, respectively. Three putative regulatory sites, two in the 5′UTR (CTTTG and CTCCTCG) and one in the 3′ UTR (CCTCTTG), are shown in bold and underlined and the entire coding region was marked with arrows. The numbers shown at the right hand side are for nucleotides and the numbers at left hand side are for deduced animo acid. Putative phosphorylation sites: S and T are circled. B) Schematic diagram of a CasAQP putative aa sequence with the position of major intrinsic protein (www.ncbi.nlm.nih.gov/Structure/cdd). C) The phylogenetic analysis of AQP 1 was carried out (http://www.phylogeny.fr) and a phylogram was constructed using the neighbor-joining method with the deduced aa sequences of the following AQP-1: C. sapidus (JQ970426); Coptotermes formosanus (BAG72254); Blattella germanica (CBY77924); Aedes aegypti (XP001656931); Daphnia pulex (EFX74648); Tribolium castaneum (XP972862); Nasonia vitripennis (XP001607940); Bombyx mori (NP001036919); Rattus norvegicus (NP036910); Xenopus laevis (NP001085391); Macaca mulatta (EHH17401); Meleagris gallopavo (XP003206949); Danio rerio (NP996942); and Homo sapiens (AAH22486). The scale bar (=0.2) represents fixed mutations per amino acid position. D) 3D structure of CasAQP-1.