Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

Assunção, Patrícia; Jaén-Molina, Ruth; Caujapé-Castells, Juli; de la Jara, Adelina; Carmona, Laura; Freijanes, Karen; Mendoza, Héctor

doi:10.1186/2046-9063-8-2

Table 1 Taxonomic classification, Culture Collection, Geographic Origin and GenBank acession numbers of the strains included in this study.

From: Molecular taxonomy of Dunaliella (Chlorophyceae), with a special focus on D. salina: ITS2 sequences revisited with an extensive geographical sampling

Former classification	New suggested classification [Reference] and comments	Culture Collection	Geographic Origin	Isolator (Date)	GenBank acession number	GenBank identifier
Subgenus Pascheria (Freshwater species)
Dunaliella lateralis Pascher &Jahoda			Nepal		AF313445	16596847
			Nepal		DQ377089	87047580
Dunaliella acidophila (Kalina) Massyuk		CCAP19/35	Freshwater; acidic sulphurous pool, Pisciarelli, Naples, Italy	Albertano (1981)	HM060646
Subgenus Dunaliella
Section Tertiolecta (Marine species. Optimum salinity < 6% NaCl)
Dunaliella tertiolecta Butcher		CCAP19/6B	Brackish; Oslo Fjord, Norway	Foyn (1928 or earlier)	HM243579*
		CCAP19/6B	Brackish; Oslo Fjord, Norway	Foyn (1928 or earlier)	AY572957	47933783
		CCAP19/27			EF473748	145587830
		CCAP19/27			AY654300	55979209
		Dtsi	Italy: Venezia		EF473730	145587823
		UTEX999	Norway: Oslofjord		AF313435	16596842
		CCMP1320	Salt flat. USA?		AF313433	16596841
		CCMP1302	Salt flat. USA?		DQ377096	87047587
		CCMP364	Salt flat. USA?		DQ377097	87047588
		FHL			DSU66956	2627284
		DCCBC5			AY686684	56578596
		SAG13.86	Norway: Oslofjord		EF473738	145587825
		ATCC30929	United Kingdom: Plymouth		EF473742	145587827
		DCCBC26			DQ224338	77955899
Dunaliella quartolecta Butcher	Dunaliella tertiolecta [this study]	CCAP19/8	Marine; Southampton, Hampshire, England	Butcher (1953)	DQ157054	77539932
Dunaliella primolecta Butcher	Dunaliella tertiolecta [this study]	UTEX1000	English Channel, Plymouth, Devon, England	Gross (1936)	AY582942	50952902
		UTEX1000	English Channel, Plymouth, Devon, England	Gross (1936)	DQ377092	87047583
	Dunaliella tertiolecta [this study]	hd8	China?		DQ116745	71482600
Dunaliella polymorpha Butcher	Dunaliella tertiolecta [this study]	CCAP19/7C	Brackish; River Crouch, Essex, England	Butcher (1954)	DQ157053	77539931
Dunaliella marítima Butcher	Dunaliella tertiolecta [this study]	SAG42.89			AY582086	51035303
Section Dunaliella (Halophilic species. Optimum salinity > 6% NaCl. Accumulates carotenes)
Dunaliella salina Teodoresco		CCAP19/18	Hypersaline; Hypersaline brines, Hutt Lagoon, Western Australia	Kaethner (1982)	AF546098	33333776
		CCAP19/18	Hypersaline; Hypersaline brines, Hutt Lagoon, Western Australia	Kaethner (1982)	EF473746	145587829
		CCAP19/25			HM140783
		UTEX1644	Point Colorado Salinas; La Paz, Baja California, Mexico	Loeblich (1967)	AF313429	16596839
		CONC006	Salar de Atacama, Chile	(1990)	AF313425	16596837
		CONC001	Laguna La Rinconada, Chile		AF546092	33333770
		CONC007	Salar de Atacama, Chile	(1990)	AF313427	16596838
		DCCBC1	Lake Tyrell, Victoria, Australia	Polle	AY549442	47499297
		DCCBC2	South Korea		AY512973	46250926
		hd6	Israel		DQ116743	71482598
			Yucatan, Mexico		AF546094	33333772
			Tanggu, China		AF546096	33333774
		AC144	Tunisia, North Africa		AY549441	47499296
	Dunaliella viridis [this study]	184.80			AY577766	49073091
		OUC66 "hd4"	China	(2005) ?	DQ116741	71482596
		OUC38 "hd3"	China	(2005) ?	DQ116740	71482595
		OUC36 "hd2"	China	(2005) ?	DQ116739	71482594
		OUC21 "hd1"	China	(2005) ?	DQ116738	71482593
		9802	China ?	(2007) ?	EF695405	151573027
		"hd5"	Inner-Mongolia	(2005)	DQ116742	71482597
	Dunaliella tertiolecta [this study]	DS18S1	Mexico?		FJ360756	213958821
	Dunaliella tertiolecta [this study]	DS18S2	Mexico?		FJ360757	213958822
	Dunaliella tertiolecta [this study]	DS18S3	Mexico?		FJ360758	213958823
	Dunaliella tertiolecta [this study]	Dsge	Belgium: Gent		EF473732	145587824
	Dunaliella viridis [ 9, 10, 27]	CCAP 19/3	Brackish; dirty salt lake, Soviet Union	Mainx	EF473744	145587828
	Dunaliella viridis [ 9, 10, 27]	UTEX200	Brackish; dirty salt lake, Soviet Union	Mainx	AF313423	16596836
		MSI-1			GQ337903	254838316
		ITC5100	Vargas, Gran Canaria, Spain	de la Jara & Mendoza (2005)	HM035353*
		ITC5101	Punta, Gran Canaria, Spain	de la Jara & Mendoza (2005)	HM035354*
		ITC5102	Tenefé, Gran Canaria, Spain	de la Jara & Mendoza (2005)	HM035355*
		ITC5103	Rio, Lanzarote, Spain	de la Jara & Mendoza (2005)	HM035356*
		ITC5104	Guatiza, Lanzarote, Spain	de la Jara & Mendoza (2005)	HM035357*
		ITC5105	Janubio, Lanzarote, Spain	de la Jara & Mendoza (2005)	HM035346*
		ITC5106	Carmen (Majo), Fuerteventura, Spain	Mendoza & Trujillano (2003)	HM035358*
		ITC5107	Añana, Álava, Spain	de la Jara & Mendoza (2005)	HM035359*
		ITC5118	île de Ré (01), France	Carmona & Mendoza (2006)	HM035348*
		ITC5122	île de Ré (05), France	Carmona & Mendoza (2006)	HM035347*
		ITC5114	La Tapa, Cádiz, Spain	de la Jara & Mendoza (2007)	HM035350*
		ITC5119	île de Ré (02), France	Carmona & Mendoza (2006)	HM035349*
	Aliquot of Dunaliella salina BCA421	ITC5003	Tenefe, Gran Canaria, Spain	Mendoza (1992)	HM035352*
Dunaliella bardawil nomen nudum Ben-Amotz & Avron	Dunaliella salina CCAP 19/30 [27], obtained from Dr. Joao Varela (Faro, Portugal)	ITC5000	Marine; salt pond, near Bardawil lagoon, North Sinai, Israel	Ben-Amotz & Avron (1976).	HM035351*
	Dunaliella salina [27]. Reinstated from SAG on April 1996	CCAP19/30	Marine; salt pond near Bardawil lagoon, North Sinai, Israel	Ben-Amotz & Avron (1976).	EU932917	205361369
	Dunaliella salina [27]	ATCC30861	Marine; salt pond near Bardawil lagoon, North Sinai, Israel	Ben-Amotz & Avron (1976)	AF313431	16596840
	Dunaliella salina [27]	UTEX2538	Marine; salt pond near Bardawil lagoon, North Sinai, Israel	Ben-Amotz & Avron (1978)	DQ377085	87047576
	Dunaliella salina [27], Dunaliella tertiolecta [this study]	SAG42.88	Marine; salt pond, near. Bardawil lagoon, North Sinai, Israel	Ben-Amotz & Avron (1976)	EF473741
	Dunaliella tertiolecta [this study]	hd7	China?		DQ116744	71482599
Dunaliella parva Lerche	Dunaliella viridis [9–11]	UTEX1983	Dead Sea	(1973)	AF313441	16596845
	Dunaliella tertiolecta [10, 11]Dunaliella quartolecta [27]	CCAP19/9	Brackish; salt marsh, Northey Island, Essex, England	Butcher (1956)	AF313439	16596844
	Dunaliella tertiolecta [10, 11]	CCMP362		Gold	AF313437	16596843
	Dunaliella marítima [27], Dunaliella viridis [this study]	SAG19-1	Marine: Lacul Sarat, Romania	Lerche (Before 1938)	DQ377091	87047582
	Dunaliella tertiolecta [this study]	hd9	China?		DQ116746	71482601
Dunaliella pseudosalina Massyuk & Radchenko		CONC010	Salar de Atacama, Chile		AF313421	16596835
Section Viridis (Halophilic species. Optimum salinity > 6% NaCl. Cells always green. Do not accumulate carotenes. Cells radially symmetrical)
Dunaliella minuta Lerche	Dunaliella tertiolecta [this study]	CCAP19/5	Marine; sand and sea water, Roscoff, France	Jowett (1967)	HM035345*
	Dunaliella tertiolecta [this study]	SAG23.86			AY582085	51035302
Dunaliella bioculata Butcher	Dunaliella tertiolecta [this study]	CCAP19/4	Brackish; salt lake, Soviet Union	Mainx	HM035344*
	Dunaliella tertiolecta [this study]	UTEX199	Brackish; salt lake, Soviet Union	Mainx	DQ157433	76097092
		UTEX199	Brackish; salt lake, Soviet Union	Mainx	DQ377086	87047577
Dunaliella viridis Massyuk		CONC002	Salar de Atacama, Chile	(1990)	AF313419	16596834
		CONC002	Salar de Atacama, Chile	(1990)	DQ377098	87047589
		SAG44.89			87047600	61200914
		SHU	China?		AY878700	58339343
		DCCBC4	Great Salt Lake, Utah, USA		AY686685	56578597
		DCCBC3	Great Salt Lake, Utah, USA		AY828227	61200913
Section Peirceinae ((Halophilic species. Optimum salinity > 6% NaCl. Cells always green. Do not accumulate carotenes. Cells bilaterally symmetrical)
Dunaliella percei Nicolai & Baas-Becking	Dunaliella tertiolecta [10, 11]	CCAP19/2	Brackish; California, USA	Nicolai (1931)	HM035343*
	Dunaliella tertiolecta [10, 11]	UTEX2192	Brackish; California, USA	Nicolai (1931)	AF313443	16596846
Unknown Dunaliella Species
Dunaliella sp.	Dunaliella tertiolecta [27]	CCAP19/23	Marine;	Pennick	HM035341*
	Dunaliella salina [27]	CCAP19/12	Brackish; North Sinai, Israel	Ginzburg (1976)	HM035342*
		CCMP367	Salt flat		DQ377087	87047578
		CCMP220	Salt flat		DQ377095	87047586
	Dunaliella tertiolecta [this study]	CCMP1923	Salt flat		DQ377094	87047585
	Dunaliella tertiolecta [this study]	CCMP1641	Salt flat		DQ377093	87047584
	Dunaliella tertiolecta [this study]	SAG19.6			AY582086	51035303
		FL1	Salt flat		DQ377099	87047590
	Dunaliella viridis [this study]	BSF1	USA: Utah, Bonneville Salt Flats	William Henley	DQ377081	87047572
	Dunaliella viridis [this study]	BSF2	USA: Utah, Bonneville Salt Flats	William Henley	DQ377082	87047573
	Dunaliella viridis [this study]	BSF3	USA: Utah, Bonneville Salt Flats	William Henley	DQ377083	87047574
	Dunaliella salina [this study]	006		A.W. Coleman, U. Brown	AF033278	2645739
	Dunaliella tertiolecta [this study]	hd10			DQ116747	71482602
	Dunaliella viridis [this study]	ABRIINW M1/1			EU927374	197290927
		ABRIINW M1/2	Iran?		EU927373	197290646
	Dunaliella salina [this study]	ABRIINW U1/1	Iran?		FJ164063	205371718
	Dunaliella viridis [this study]	ABRIINW U2/1	Iran?		FJ164064	205371719
		SPMO112-3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377103	87047594
		SPMO201-3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377110	87047601
		SPMO128-2	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377105	87047596
		SPMO109-1	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377105	87047596
		SPMO112-4	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377104	87047595
		SPMO207-3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377115	87047606
		SPMO200-3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377107	87047598
		SPMO201-4	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377111	87047602
		SPMO201-5	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377112	87047603
		SPMO201-6	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377113	87047604
		SPMO112-1	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377101	87047592
		SPMO112-2	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377102	87047593
		SPMO300-4	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377118	87047609
		SPMO210-3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377116	87047607
	Dunaliella viridis [this study]	SPMO200-8	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377108	87047599
	Dunaliella viridis [this study]	SPMO601-1	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377121	87047612
	Dunaliella viridis [this study]	SPMO200-2	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377106	87047597
	Dunaliella viridis [this study]	SPMO201-2	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377109	87047600
	Dunaliella viridis [this study]	SPMO202-4	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377114	87047605
	Dunaliella viridis [this study]	SPMO300-5	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377119	87047610
	Dunaliella viridis [this study]	SPMO600-1	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377120	87047611
	Dunaliella viridis [this study]	SPMO BP3	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377122	87047613
	Dunaliella viridis [this study]	SPMO 980625-IE	Salt flat, USA: Oklahoma, Salt Plains National Wildlife Refuge		DQ377123	87047614
Other groups
Chlamydomonas reinhardtii		SAG11-31			AJ749628	111073405
Chlamydomonas allensworthii		isolate 7			AF326855	13274452
Oedogonium nodulosum					DQ078301	71482662
Bulbochaete rectangularis var. hiloensis		UTEX954	Catawmont, Massachusetts, USA	Cook 1962	AY962677	62183531
Astrephomene gubernaculifera		UTEX2479			AGU66932	2982755
Pandorina morum		EP3			AF378359	14165185
Eudorina elegans		UTEX 1192			AF098173	4007503
Yamagishiella unicocca		UTEX2431			AF375785	14091689
Phacotus lenticularis		970805-20			AY009933	18025134
Lobochlamys culleus		UTEX1057	Maxville, Florida, USA	Smith	CCU66946	2627275

Asterisks indicate the strains sequenced in this study. Underlined strains correspond to identical isolates stored in different culture collections.
Acronyms: Culture Collection of Algae and Protozoa, UK (CCAP); Sammlug von Algenkulturen, Germany (SAG); University of Texas Culture Collection of Algae, USA (UTEX); American Type Culture Collection, USA (ATCC); Dunaliella Culture Collection at Brooklyn College, USA (DCCBC); Provasoli-Guillard National Centre for the culture of Marine Phytoplankton, USA (CCMP); Universidad de Concepción, Chile (CONC); Banco Canario de Algas, Spain (BCA) ; Instituto Tecnológico de Canarias, Spain (ITC).

Back to article page

Aquatic Biosystems