From: Molecular (PCR-DGGE) versus morphological approach: analysis of taxonomic composition of potentially toxic cyanobacteria in freshwater lakes
Primer
Sequence (5′ to 3′)
Melting temperatures (°C)
Reference
ITS
(GC)CSIF
G(T/C)CACGCCCGAAGTC(G/A)TTAC
53.8-57.9
[7]
373R
CTAACCACCTGAGCTAAT
45.8
[7, 27]
mcy A
(GC)mcyA-Cd1F
AAAATTAAAAGCCGTATCAAA
42.6 (84.1 with CG clamp)
[8, 28]
mcyA-Cd1R
AAAAGTGTTTTATTAGCGGCTCAT
50.6
