Comparison of four phaC genes from Haloferax mediterranei and their function in different PHBV copolymer biosyntheses in Haloarcula hispanica

Table 2 PHA accumulation in Har. hispanic a strains^a.

Har. hispanica Strain	CDW	PHA content	PHA concentration	PHA composition (mol %)
	(g/L)	(wt %)	(g/L)	3HB (C4)	3HV (C5)
Wild type	4.70 ± 0.23	12.26 ± 0.52	0.58 ± 0.03	96.82 ± 0.35	3.18 ± 0.35
PHB-1 (pWL3E)	4.90 ± 0.12	ND	ND	ND	ND
PHB-1 (pWL3EC)	5.27 ± 0.61	17.33 ± 0.04	0.99 ± 0.11	96.86 ± 0.13	3.14 ± 0.13
PHB-1 (pWL3EC1)	6.00 ± 0.07	15.61 ± 0.06	0.94 ± 0.01	95.94 ± 0.50	4.06 ± 0.03
PHB-1 (pWL3EC2)	5.58 ± 0.21	ND	ND	ND	ND
PHB-1 (pWL3EC3)	5.31 ± 0.14	10.02 ± 1.06	0.53 ± 0.07	98.05 ± 0.20	1.95 ± 0.20

^a The cells were cultured in MG medium at 37°C for 96 h. For each strain, three samples were cultured and analyzed separately at the same time. Data are shown as the mean ± standard deviation; n = 3. ND, not detectable.