Culture independent molecular analysis of bacterial communities in the mangrove sediment of Sundarban, India

Aquatic Biosystems

Table 1 Summary of the 16S rRNA gene sequences identified in the D16S_pMOS and DUni_pMOS clone libraries

Bacterial division	Number of sequence types	Number of sequence types in D16S_pMOS library (%)	Number of sequence types in DUni_pMOS library (%)	Number of total clones (%)	Sequence similarity to the closest relatives^c(%)
Proteobacteria	86	29 (58%)^a	57 (71%)^a	66.1	83-100
Alpha	6	1 (3.5%)^b	5 (8.7%)^b	4.6	87-96
Beta	7	4 (14%)^b	3 (5.2%)^b	5.4	92-99
Gamma	61	17 (59%)^b	44 (77%)^b	47	86-100
Delta	10	6 (21%)^b	4 (7%)^b	7.7	83-99
Unassigned proteobacteria	2	1 (2%)^b	1 (1.25%)^b	1.5	91-94
CFB group	1	1 (2%)^a	0	0.8	93
Actinobacteria	2	0	2 (2.5%)^a	1.53	87-98
Planctomycetes	4	2 (4%)^a	2 (2.5%)^a	3	86-92
Firmicutes	1	1 (2%)^a	0	0.8	92
Chloroflexi	2	0	2 (2.5%)^a	1.53	89-98
Gemmatimonadates	2	1(2%)^a	1 (1.25%)^a	0.75	94-96
Acidobacteria	1	0	1 (1.25%)^a	0.8	91
Marie eubacterium	1	1 (2%)^a	0	0.8	89
Bacterial candidate division OP8	1	0	1 (1.25%)^a	0.8	87
Uncultured	24	11 (22%)^a	13 (16%)^a	18.5	86-99
Unidentified	5	4 (8%)^a	1 (1.25%)^a	3.9	91-98
Total	130	50	80	--	83-100

"a" % of clones among all the 130 clones selected from D16S_pMOS and DUni_pMOS libraries
"b" % of clones among the proteobacterial clones under respective library
"c" Closest relatives as determined by the BLAST analysis