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Figure 2 | Saline Systems

Figure 2

From: Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 1: an ovarian stage dependent involvement

Figure 2

Evaluation and optimization of the hepatopancreas incubation experiment. A) Incubation period had no effect on the stability of VtG mRNA in hepatopancreas fragments of females at early ovarian stage 2 (E2). Bars, normalized VtG mRNA; dashed line, total RNA. MIH had a more pronounced decreasing effect on VtG mRNA levels than CHH (0 – 20 nM) in hepatopancreas fragments of females at E2 incubated for 1 and 6 h, B) Incubation with 0 to 5 nM MIH and C) Incubation with 0 to 20 nM CHH. circles, 1 h incubation; squares, 6 h incubation. Data are presented as mean ± SEM (N = 3).

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Aquatic Biosystems

ISSN: 2046-9063