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Figure 1 | Saline Systems

Figure 1

From: Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 1: an ovarian stage dependent involvement

Figure 1

MIH and CHH levels in the hemolymph and mRNAs in the XO of pre- and mid-vitellogenic females (ovarian stage 1 and 3). A) hemolymph titers and B) mRNA a) MIH, b) CHH. MIH and CHH neuropeptides in the hemolymph were extracted with 40% isopropanol for the extraction of CHH and 60% isopropanol for the extraction of MIH followed by a specific RIA in 10 females at each stage 1 or 3. MIH and CHH mRNA levels were determined using QPCR in 17 females at stage 1 and 21 females at stage 3 and are presented as copies/20 ng total RNA. Results are presented as mean ± SEM (N = 10). **, = P ≤ 0.01.

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