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Figure 7 | Saline Systems

Figure 7

From: Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 2: novel specific binding sites in hepatopancreas and cAMP as a second messenger

Figure 7

Binding sites of [125I] MIH in the membranes of female's hepatopancreas and juvenile YO have a similar molecular mass of ~51 kDa, which is different from that of [125I] CHH in the hepatopancreas. A) J-YO (100 μg); B) female hepatopancreas at ovarian stage 3 (200 μg). Membranes were incubated without (total binding, Tb) or with unlabeled MIH or CHH in an excess of 1 or 10 pmol, specified at the top of the lane. YO membranes were resolved on a 4–15% SDS-PAGE and hepatopancreas membranes on a 10% SDS-PAGE. Labeled ligand and receptor complexes are indicated with arrows.

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