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Figure 6 | Saline Systems

Figure 6

From: Molt-inhibiting hormone stimulates vitellogenesis at advanced ovarian developmental stages in the female blue crab, Callinectes sapidus 2: novel specific binding sites in hepatopancreas and cAMP as a second messenger

Figure 6

cGMP analog mimics the effects of CHH and cAMP analog mimics the effect of MIH on VtG mRNA and hnVtG RNA in the hepatopancreas fragments of stage E2 females, respectively. Hepatopancreas fragments were incubated with 10 μM 8-Bromo- cGMP or 8-Bromo- cAMP followed by QPCR analysis to detect changes in hnVtG (Black) and VtG mRNA (Grey). The results are presented as mean ± SEM of % of control (N = 4). *, P ≤ 0.05; **, P ≤ 0.01.

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