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Table 1 Oligonucleotides used for real-time PCR to amplify halophilic dsrA genes closely related to Desulfocella halophila.

From: Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds

Primer paira Sequence (5'-3')b Primer binding sitec Product size (bp)
RH-halo-1F GTTCTTcTtGGTACAAGAACAGA 192–214 144
RH-halo-1R GCATGAGTATTCACATCTT 317–335  
RH-halo-1F GTTCTTcTtGGTACAAGAACAGA 192–214 187
RH-halo-2R GGAATTCCTGTGTCAAgAAaTGA 356–378  
  1. a. (F) and (R) correspond to forvard and reverse primers, respectively.
  2. b. Bases that do not match appropriate sequences are shown as lowercase letters.
  3. c. Positions within the Desulfocella halophila DSM 11763 (AF418200; Friedrich, 2002) dsrA open reading frame.