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Table 1 Oligonucleotides used for real-time PCR to amplify halophilic dsrA genes closely related to Desulfocella halophila.

From: Long-term surveillance of sulfate-reducing bacteria in highly saline industrial wastewater evaporation ponds

Primer paira

Sequence (5'-3')b

Primer binding sitec

Product size (bp)

RH-halo-1F

GTTCTTcTtGGTACAAGAACAGA

192–214

144

RH-halo-1R

GCATGAGTATTCACATCTT

317–335

 

RH-halo-1F

GTTCTTcTtGGTACAAGAACAGA

192–214

187

RH-halo-2R

GGAATTCCTGTGTCAAgAAaTGA

356–378

 
  1. a. (F) and (R) correspond to forvard and reverse primers, respectively.
  2. b. Bases that do not match appropriate sequences are shown as lowercase letters.
  3. c. Positions within the Desulfocella halophila DSM 11763 (AF418200; Friedrich, 2002) dsrA open reading frame.
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