Figure 2
Genetic organization of genes potentially involved in the biosynthesis of glutamine and glutamate in Halobacillus halophilus. (A) Glutamate dehydrogenase (gdh). Two ORFs encoding a putative glutamate dehydrogenase (gdh1 and gdh2) could be identified. orf1: L-asparaginase, orf2: pyridine nucleotide-disulfide oxidoreductase family protein (thioredoxin reductase), orf3: D-alanyl-D-alanine ligase A, orf4: negative regulator of genetic competence, orf5: unknown, orf6: enoyl-[acyl-carrier protein] reductase, orf7: unknown. (B) Glutamate synthase (glt). The glutamate synthase is a heterodimer, encoded by gltA (large subunit) and gltB (small subunit). In the genome of Halobacillus halophilus only one ORF could be identified encoding GltA whereas two ORFs were found encoding GltB (gltB1 and gltB2). orf1: catalase, orf2: L-aminopeptidase/D-esterase, orf3: dihydropyrimidine dehydrogenase, orf4: β-alanine synthase. (C) Glutamine synthetase (glnA). orf1: hypothetical conserved protein, glnR: transcriptional regulator, orf2: aluminum resistance protein, orf3: GTP-binding protein, orf4: spore formation protein, orf5: ABC transporter protein, orf6: oxidoreductase, orf7: putative dehydrogenase, orf8: conserved protein, orf9: conserved hypothetical protein/GTPase of unknown function, orf10: hypothetical protein.