Skip to main content

Table 1 The list of primer sequences that was used for cloning of TPS gene and QRT-PCR

From: A trehalose 6-phosphate synthase gene of the hemocytes of the blue crab, Callinectes sapidus: cloning, the expression, its enzyme activity and relationship to hemolymph trehalose levels

 

Primer sequences (5'-3')

TPS dR1

TCDCCRGCDCCRGCRAADGG

TPS dR2

ATYTGRCARGCSACRAAYTC

TPS 3dF1

TTYGAYTCYTAYTAYAAYGG

TPS 3dF2

TTYTGGCCNYTNTTYCAYTCYATGCC

TPSF1

ATGCCTGACAGAGCAACATTTCAG

TPSF2(=QF1)

ATGTTGGTGGAACACAATTCAAGGAC

TPSR1

TACAGAAGAGTCTCGGTAGAATGCA

TPSR2(=QR)

CTTTGTATAATCTAACCGATCCACTC

TPSR3

GCACGGAGTCTGGGTGGCTCTCA

  1. 'd' represents degenerate primers. Two forward primers of TPS F1 and F2 were used for 3' RACE and three of TPSR1, R2, and R3 were for 5'RACE. Primers of QF1 and QR1 were used for QRT-PCR analysis.