Table 2 Primers used for the rapid amplification of cDNA ends (RACE)
From: Heterologous ectoine production in Escherichia coli: By-passing the metabolic bottle-neck
Primer | Sequence | Target |
|---|---|---|
Race A1 | 5'-GGAATGAAGGCCGTTACGAA-3' | ectA (RT primer) |
Race A2 | 5'-ACGATTGAATCGACGGAACC-3' | ectA (PCR primer) |
Race B1 | 5'-TGCCGTGGAAGCCATTAGTA-3' | ectB (RT primer) |
Race B2 | 5'-CCGTCTTCCTGAATATAGGT-3' | ectB (PCR primer) |
Race C1 | 5'-ACGCCTGGTTCCAGCTGATA-3' | ectC (RT primer) |
Race C2 | 5'-CCGGCACGAATAATTGTGTC-3' | ectC (PCR primer) |
dG 15 | 5'-TAGATCTAGAGCTCGGGGGGGGGGGGGGG-3' | oligo-dC tail |