Heterologous ectoine production in Escherichia coli: By-passing the metabolic bottle-neck

Aquatic Biosystems

Table 1 Aspartate kinase activity

Strain	specific activity [nmol (min mg protein)^-1]
	No addition	Lys/Thr
E. coli DH5α pOSM12	5.4 ± 0.2	2.4 ± 0.1
E. coli DH5α pOSM2	5.2 ± 0.2	2.2 ± 0.1
E. coli DH5α pAKECT1	10.0 ± 0.4	10.1 ± 0.5

Specific aspartate kinase activity in recombinant ectoine producing E. coli pAKECT1 and its inhibition by L-lysine and L-threonine at a final concentration of 10 mM in the assay mixture. Mean values and standard deviations are based on three independent experiments.