Figure 6

Influence of feed-back inhibitors and precursors. Intracellular ectoine concentrations of E. coli DH5α pOSM12 in minimal medium MM63 at 3% NaCl, as influenced by supplementation with the feedback-inhibitors and transcriptional repressors L-lysine (lys), L-threonine (thr) and L-methionine (met) (each 1 mM), or with the substrate L-aspartate (1 mM) and its precursor fumarate (1 mM). Control experiments were performed in the absence of regulating amino acids.