Figure 5

Maximum growth rates. Maximum growth rates [h^-1] of the recombinant ectoine producer E. coli DH5α pOSM12 (▲) and of the control strain E. coli DH5α pHSG575, with (●) and without (○) supplementation of 2 mM ectoine at salinities of between 1% and 5% NaCl in minimal medium MM63. The novel construct pAKECT1 (■) employing deregulated aspartate kinase from C. glutamicum (induced with 0.5 mM IPTG) displayed improved growth at salinities above 3% NaCl. Mean values and standard deviations are based on three independent experiments.