Figure 4
From: Proteomics with a pinch of salt: A cyanobacterial perspective
An overview of a proteomic procedure with iTRAQ reagents. Proteins are extracted from four phenotypes which are to be compared and trypsin digested. The resulting peptides are labelled with isobaric tags and then pooled for fractionation and analysis at the MS/MS stage on the mass spectrometer. Isobaric tag intensity corresponds to peptide abundance and is used for protein quantitation.