Figure 2

(A) A simple salt removal strategy for proteomic experiments using gel electrophoresis for protein fractionation. Desalting steps are in green boxes, example techniques in circular boxes and parameters in diamond boxes. N.B. For halophilic organisms the wash step with no salt buffer must be performed at the protein extraction stage. (B) Euhalothece desalting optimisation tests. 7 cm² gels, pI 3–10. i) No desalting steps ii) TCA/acetone precipitation only iii) Desalting column not used iv) External salt not washed v) Complete desalting procedure followed.