Table 3 PCR primers Primers used in PCR for constructing deletions and for screening uvr alleles. All listed 5'→3'. Engineered restriction sites underlined

Primers for constructing deletions:

uvrA1 forward

GGGGGTACCGTATTTGTTCGGCACGAGGT

uvrA1 reverse

GGGTCTAGACTCTTCGCTCATTGGGAGAG

uvrA2 forward

GGGTCTAGACTCTCGCGGCTCTGTCTC

uvrA2 reverse

GGGAAGCTTACCGTCTCAGTGGTGGTGTC

uvrB1 forward

GGGGGTACCAGGAACGCGACCACTACG

uvrB1 reverse

GGGTCTAGAGCTGGCGTCACTCATTACAC

uvrB2 forward

GGGTCTAGAGAAACCGAGGACTGGTGAGA

uvrB2 reverse

GGGAAGCTTGGGAACACGAAGATGAGGAA

uvrC1 forward

GGGGGTACCGTACGTGGGTGTGATGAACG

uvrC1 reverse

GGGTCTAGATTCACGACTGTCTCCACGTC

uvrC2 forward

GGGTCTAGAAGAACGACGACTACGCGAAC

uvrC2 reverse

GGGAAGCTTACGTCTCGGAGTACCAGCAG

Primers for screening deletions by PCR

uvrA up

AATGTCGTAGTCGGCCATGT

uvrA down

CACAGCCCCGAGACAGAG

uvrB up

GGCCTACGACGAGTACACC

uvrB down

TGAAAAGCGTTGGTTTCTCC

ura3 up

CTTCCGGAGGACGTACAGG

ura3 down

CGTACTGGGCGTTCCACT

bla up

TTTGCCTTCCTGTTTTTGCT

bla down

TTGCCGGGAAGCTAGAGTAA