Skip to main content

Advertisement

Table 7 Bacterial strains used in this study

From: NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?

Strain Genotype reference
Escherichia coli DH5α F- 80dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk- mk+)deoR supE44 λ- thi-1 gyrA96 relA1 [36]
Escherichia coli DH5α pUCHelNhaD see above, plus nhaD on pUC18 under regulation of lacI This study
Escherichia coli EP432 melBLid, ΔnhaB1ΔnhaA1, ΔlacZY, thr1 [11]
Escherichia coli EP432 pUCHelNhaD see above, plus nhaD on pUC18 under regulation of lacI This study
Escherichia coli Knabc TG1 nhaA::KmR nhaB::EmR chaA::CmR [12]
Escherichia coli KNabc pUCHelNhaD see above, plus nhaD on pUC18 under regulation of lacI This study
Escherichia coli NM81 melBLid, nhaB+ΔnhaA1, kan+, ΔlacZY, thr1 [13]
Escherichia coli NM81 pUCHelNhaD see above, plus nhaD on pUC18 under regulation of lacI this study
Escherichia coli XL1blue recA1 endA1 gyrA96 thi hsdR17 (rk- mk+) supE44 relA1 λ-lac- [F' proAB lacIqZΔM15 Tn10(tet)] [37]
Halomonas elongata DSM2581T Wild type [1]
  1. E. coli strains NM81 and EP432 were kindly provided by Prof. Dr. E. Padan.
  2. E. coli strain KNabc was kindly provided by Prof. Dr. T. Tsuchia