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Table 7 Bacterial strains used in this study

From: NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?

Strain

Genotype

reference

Escherichia coli DH5α

F- 80dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk- mk+)deoR supE44 λ- thi-1 gyrA96 relA1

[36]

Escherichia coli DH5α pUCHelNhaD

see above, plus nhaD on pUC18 under regulation of lacI

This study

Escherichia coli EP432

melBLid, ΔnhaB1ΔnhaA1, ΔlacZY, thr1

[11]

Escherichia coli EP432 pUCHelNhaD

see above, plus nhaD on pUC18 under regulation of lacI

This study

Escherichia coli Knabc

TG1 nhaA::KmR nhaB::EmR chaA::CmR

[12]

Escherichia coli KNabc pUCHelNhaD

see above, plus nhaD on pUC18 under regulation of lacI

This study

Escherichia coli NM81

melBLid, nhaB+ΔnhaA1, kan+, ΔlacZY, thr1

[13]

Escherichia coli NM81 pUCHelNhaD

see above, plus nhaD on pUC18 under regulation of lacI

this study

Escherichia coli XL1blue

recA1 endA1 gyrA96 thi hsdR17 (rk- mk+) supE44 relA1 λ-lac- [F' proAB lacIqZΔM15 Tn10(tet)]

[37]

Halomonas elongata DSM2581T

Wild type

[1]

  1. E. coli strains NM81 and EP432 were kindly provided by Prof. Dr. E. Padan.
  2. E. coli strain KNabc was kindly provided by Prof. Dr. T. Tsuchia