MM Na+/mM Li+ | pH 7 | pH 7.5 | pH 8 | pH 8.5 | pH 9 |
---|
pUC18 control | | | | | |
500/0 | +++ | +++ | ++ | + | - |
400/100 | ++ | ++ | + | + | - |
300/200 | + | + | + | (+) | - |
200/300 | - | - | - | - | - |
pUCHelNhaD | | | | | |
500/0 | +++ | +++ | ++ | + | - |
400/100 | ++ | ++ | + | (+) | - |
300/200 | + | + | (+) | - | - |
200/300 | - | - | - | - | - |
- Growth of E. coli strains DH5α pUC18 and DH5α pUCHelNhaD on solid and in liquid MM63 minimal medium, low-phosphate version supplemented with NaCl and LiCl as indicated. Growth quality judged from colony size and microscopic behaviour: +++ very good, ++ good, + poor, (+) mere survival of cells, - no growth.