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Table 5 Li+-sensitivity of E. coli DH5α

From: NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?

Culture

Strain

growth rate/h-1

rel. growth

lag phase/h

OD600 max

MM63, NaCl

NhaD

0.37 ± 0.03

1

2

2.8 ± 0.2

 

Control

0.35 ± 0.03

1

2

3.0 ± 0.2

MM63, NaCl/LiCl

NhaD

0.20 ?

0.7

?

0.3 ?

 

Control

0.28 ± 0.03

1

2

0.7 ± 0.1

  1. Growth of E. coli strains DH5α pUC18 (control) and DH5α pUCHelNhaD (NhaD) at 500 mM (3% (w/v)) NaCl and 300 mM NaCl/200 mM LiCl in MM63 minimal medium. Growth rates, duration of lag phase and maximum OD(600 nm) depending on culture and strain are summarized. Relative growth rate is given for expression strain compared to the control at same salinity.