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Figure 2 | Saline Systems

Figure 2

From: NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?

Figure 2

H. elongata nhaD sequence. Part of the DNA sequence of H. elongata nhaD and regions upstream and downstream of the coding region: Bases are numbered on the left (numbering according to full sequence as found in EMBL nucleotide sequence database: AM167899), the deduced AA sequence is below the codons and numbered on the right. -35a, -10a putative rpoH dependent promoter, SDa putative Shine-Dalgarno sequence after rpoH and two putative start codons ATG. -35b and -10b putative σ70 housekeeping promoter, SDb alternative Shine-Dalgarno sequence and atgpossible start codon. Note that the last start codon is the only one usable for expression under control of both putative promoters. tgastopp codons in the first *, second *2 and third *3 reading frame. →← inverted repeat, the downstream sequence lacks an AT-rich motive, therefore this is probably not significant for transcription termination. Note also that there are two possible starting points for transcription and translation, yielding proteines of 483 or 412 residues. Translation starting after SDb would yield only the 412 AA protein. On the other hand SDa would only be functional only for the rpoH dependent promoter.

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