Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm)

Echigo, Akinobu; Hino, Miki; Fukushima, Tadamasa; Mizuki, Toru; Kamekura, Masahiro; Usami, Ron

doi:10.1186/1746-1448-1-8

Table 1 Strains isolated from ordinary soil samples on agar plates containing 20% NaCl.

From: Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm)

Strain No.	Sampling site	Pig.	NaCl (M)		pH		Similarity	Tentatively assigned to
			Range	Optimum	Range	Optimum	(%)
3	Omiya, S	-	0.9–2.6	0.9–1.7	6.5–10.0	8.5–9.5	100	B. haloalkaliphilus (AJ238041)
12	Showa, S	-	0.9–4.3	1.7–2.6	6.5–10.0	8.5–9.5	100	B. haloalkaliphilus
27	Takasaki, G	-	1.7–3.4	1.7–2.6	6.5–10.0	8.5–9.5	100	B. haloalkaliphilus
29	Kamagaya, C	-	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	100	B. haloalkaliphilus
1	Wako, S	-	0.9–4.3	1.7–2.6	6.5–10.0	8.5–9.5	99.8	B. haloalkaliphilus
28	Sakado, S	-	1.7–2.6	1.7–2.6	6.5–10.0	8.5–9.5	99.8	B. haloalkaliphilus
7	Higashichichibu, S	-	0.9–1.7	0.9–1.7	6.5–10.0	8.5–9.5	99.6	B. haloalkaliphilus
8	Higashichichibu, S	-	0.9–2.6	0.9–1.7	6.5–10.0	8.5–9.5	99.6	B. haloalkaliphilus
2	Yachiyo, C	-	0–3.4	0.9–1.7	6.5–10.0	8.5–9.5	98.4	B. haloalkaliphilus
18	Kawagoe, S	-	0.9–4.3	1.7–2.6	6.5–10.0	8.5–9.5	98.0	B. haloalkaliphilus
31	Okabe, S	-	0–3.4	1.7–2.6	6.5–10.0	6.5–7.5	97.2	F. milosensis (AJ238042)
19	Wako, S	-	0.9–4.3	1.7–2.6	6.5–10.0	8.5–9.5	94.0	F. milosensis
9	Shiki, S	-	0–3.4	0.9–1.7	6.5–10.0	6.5–7.5	96.1	G. halotolerans (AB101591)
17	Urawa, S	-	0–4.3	0.9–1.7	6.5–10.0	8.5–9.5	91.4	B. agaradhaerens (X76445)
22	Kawagoe, S	-	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	88.8	H. trueperi (AJ310149)
14	Kawagoe, S	Y	0.9–3.4	1.7–2.6	6.5–10.0	8.5–9.5	88.7	H. trueperi
13	Kawagoe, S	Y	0.9–3.4	1.7–2.6	6.5–10.0	8.5–9.5	88.2	H. trueperi
25	Okegawa, S	-	1.7–3.4	1.7–2.6	6.5–10.0	8.5–9.5	88.0	H. trueperi
10	Tsurugashima, S	B	1.7–2.6	1.7–2.6	6.5–10.0	8.5–9.5	88.0	'B. nitritophilus' (AJ309562)
11	Showa, S	Y	0–3.4	1.7–2.6	6.5–10.0	8.5–9.5	87.8	'B. nitritophilus'
16	Iruma, S	B	1.7–3.4	1.7–2.6	6.5–10.0	8.5–9.5	87.7	'B. nitritophilus'
4	Matsubushi, S	-	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	87.5	'B. nitritophilus'
21	Omiya, S	B	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	87.5	'B. nitritophilus'
15	Omiya, S	B	1.7–3.4	1.7–2.6	6.5–10.0	8.5–9.5	87.3	'B. nitritophilus'
5	Kawasaki, K	B	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	86.9	'B. nitritophilus'
24	Soka, S	-	0–4.3	0.9–1.7	6.5–10.0	8.5–9.5	86.9	'B. nitritophilus'
30	Okabe, S	-	1.7–4.3	1.7–2.6	6.5–10.0	8.5–9.5	87.3	'Pc. psychrotoleratus' (AF324659)
61	Omiya, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.3	F. milosensis (AJ238042)
66	Koga, I	-	0–2.6	0–0.9	6.5–8.0	6.5–7.5	97.1	F. milosensis
173	Tsurugashima, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.1	F. milosensis
106	Showa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.0	F. milosensis
112	Yachiyo, C	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	97.0	F. milosensis
113	Yachiyo, C	Y	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.0	F. milosensis
117	Niiza, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.0	F. milosensis
168	Tsurugashima, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.0	F. milosensis
176	Kawagoe, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.0	F. milosensis
70	Okegawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.9	F. milosensis
105	Kashiwa, C	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.9	F. milosensis
116	Niiza, S	Y	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.9	F. milosensis
172	Ageo, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.9	F. milosensis
69	Kitamoto, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.7	F. milosensis
170	Asaka, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.7	F. milosensis
60	Kasukabe, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.5	F. milosensis
185	Urawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.4	F. milosensis
74	Hanyu, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	96.3	G. halotolerans (AB101591)
75	Hanyu, S	-	0–2.6	0–0.9	6.5–8.0	6.5–7.5	96.3	G. halotolerans
76	Katsushika, T	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.3	G. halotolerans
102	Omiya, S	-	0–2.6	0–0.9	5.5–8.0	6.5–7.5	96.3	G. halotolerans
72	Kawagoe, S	-	0–2.6	0–0.9	6.5–8.0	6.5–7.5	96.1	G. halotolerans
77	Katsushika, T	-	0–3.4	1.7–2.6	6.5–8.0	6.5–7.5	94.8	H. karajensis (AJ486874)
51	Okegawa, S	-	0–3.4	1.7–2.6	6.5–8.0	6.5–7.5	100	H. litoralis (X94558)
54	Fujimi, S	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	100	H. litoralis
92	Fujimi, S	Y	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	100	H. litoralis
188	Ageo, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	100	H. litoralis
195	Fujimi, S	-	0–4.3	0–0.9	6.5–8.0	6.5–7.5	99.5	H. litoralis
200	Kawagoe, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	99.4	H. litoralis
91	Itabashi, T	-	0.9–3.4	0.9–2.6	6.5–8.0	6.5–7.5	98.8	H. litoralis
130	Sakado, S	-	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	98.8	H. litoralis
196	Urawa, S	Y	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	98.6	H. litoralis
152	Kawagoe, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	97.2	H. litoralis
136	Urawa, S	-	0–3.4	1.7–2.6	6.5–8.0	6.5–7.5	97.0	H. litoralis
97	Higashichichibu, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	96.7	H. litoralis
154	Ina, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.6	H. litoralis
78	Shiki, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.4	H. litoralis
132	Urawa, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.4	H. litoralis
180	Toda, S	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.4	H. litoralis
107	Showa, S	Y	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.3	H. litoralis
143	Nerima, T	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	96.3	H. litoralis
162	Kawagoe, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.3	H. litoralis
86	Wako, S	-	0–2.6	0.9–2.6	6.5–8.0	6.5–7.5	96.2	H. litoralis
88	Fujimi, S	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.2	H. litoralis
119	Toride, I	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.2	H. litoralis
131	Iruma, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.2	H. litoralis
133	Urawa, S	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.2	H. litoralis
164	Ota, T	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.2	H. litoralis
192	Tama, T	-	0–4.3	0.9–1.7	6.5–8.0	6.5–7.5	96.2	H. litoralis
55	Wako, S	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.1	H. litoralis
68	Okegawa, S	-	0–3.4	1.7–2.6	6.5–8.0	6.5–7.5	96.1	H. litoralis
84	Higashimurayama, T	Y	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.1	H. litoralis
85	Toshima, T	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.1	H. litoralis
95	Soka, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.1	H. litoralis
100	Higashichichibu, S	-	0–3.4	0.9–2.6	5.5–8.0	6.5–7.5	96.1	H. litoralis
104	Omiya, S	Y	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.1	H. litoralis
124	Koto, T	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.1	H. litoralis
144	Sakado, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.1	H. litoralis
145	Sakado, S	-	0–4.3	0.9–1.7	6.5–8.0	6.5–7.5	96.1	H. litoralis
151	Tsurugashima, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.1	H. litoralis
184	Yoshimi, S	Y	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.1	H. litoralis
203	Omiya, S	-	0.9–3.4	0.9–1.7	5.5–8.0	6.5–7.5	96.1	H. litoralis
56	Fujimi, S	-	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
62	Higashimatsuyama, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
65	Yachiyo, C	-	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
83	Nerima, T	Y	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
89	Fujimi, S	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.0	H. litoralis
109	Kyowa, I	Y	0–4.3	0.9–2.6	6.5–8.0	6.5–7.5	96.0	H. litoralis
120	Toride, I	-	0.9–3.4	0.9–2.6	6.5–8.0	6.5–7.5	96.0	H. litoralis
128	Tsurugashima, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
146	Kawagoe, S	-	0–2.6	0–0.9	5.5–8.0	6.5–7.5	96.0	H. litoralis
175	Tsurugashima, S	-	0–4.3	0–0.9	6.5–8.0	6.5–7.5	96.0	H. litoralis
182	Niiza, S	-	0.9–4.3	0.9–1.7	6.5–8.0	6.5–7.5	96.0	H. litoralis
189	Ageo, S	-	0–2.6	0.9–1.7	5.5–8.0	6.5–7.5	96.0	H. litoralis
57	Itabashi, T	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	95.9	H. litoralis
63	Nerima, T	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.9	H. litoralis
110	Kyowa, I	Y	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	95.9	H. litoralis
127	Tsurugashima, S	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.9	H. litoralis
187	Koga, I	-	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.9	H. litoralis
80	Koshigaya, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.8	H. litoralis
155	Omiya, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.8	H. litoralis
166	Tsurugashima, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.8	H. litoralis
202	Omiya, S	-	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	95.8	H. litoralis
59	Higashichichibu, S	Y	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.7	H. litoralis
79	Shiki, S	Y	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.7	H. litoralis
159	Omiya, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.7	H. litoralis
156	Omiya, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.6	H. litoralis
178	Urawa, S	-	0–4.3	0.9–1.7	6.5–8.0	6.5–7.5	95.6	H. litoralis
165	Warabi, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	95.2	H. litoralis
52	Omiya, S	Y	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	100	H. trueperi (AJ310149)
64	Shiki, S	-	0.9–3.4	1.7–2.6	5.5–8.0	6.5–7.5	100	H. trueperi
67	Okegawa, S	-	0.9–3.4	1.7–2.6	6.5–8.0	6.5–7.5	100	H. trueperi
82	Omiya, S	-	1.7–2.6	1.7–2.6	6.5–8.0	6.5–7.5	100	H. trueperi
96	Soka, S	-	0.9–3.4	1.7–2.6	6.5–8.0	6.5–7.5	100	H. trueperi
98	Higashimatsuyama, S	Y	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	100	H. trueperi
99	Higashimatsuyama, S	Y	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	100	H. trueperi
111	Kyowa, I	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	100	H. trueperi
114	Shiki, S	-	0.9–3.4	0.9–1.7	5.5–8.0	6.5–7.5	100	H. trueperi
118	Toride, I	Y	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	100	H. trueperi
122	Urawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	100	H. trueperi
150	Tsurugashima, S	Y	0–3.4	0–0.9	6.5–8.0	6.5–7.5	99.8	H. trueperi
181	Higashimatsuyama, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	99.7	H. trueperi
157	Nagareyama, C	-	0–4.3	1.7–2.6	6.5–8.0	6.5–7.5	99.6	H. trueperi
58	Kamagaya, C	Y	0–3.4	0–0.9	5.5–8.0	6.5–7.5	99.5	H. trueperi
53	Kamagaya, C	Y	0–3.4	0–0.9	5.5–8.0	6.5–7.5	99.4	H. trueperi
87	Kawasaki, K	Y	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	99.3	H. trueperi
94	Soka, S	-	0–3.4	0.9–2.6	6.5–8.0	6.5–7.5	99.3	H. trueperi
153	Warabi, S	Y	0–3.4	0–0.9	6.5–8.0	6.5–7.5	99.2	H. trueperi
163	Kawagoe, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	99.2	H. trueperi
142	Kawagoe, S	Y	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	99.0	H. trueperi
140	Urawa, S	Y	0–4.3	0.9–2.6	5.5–8.0	6.5–7.5	98.9	H. trueperi
139	Urawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	98.6	H. trueperi
81	Kasukabe, S	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	98.3	H. trueperi
129	Tsurugashima, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	98.3	H. trueperi
193	Wako, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	98.1	H. trueperi
90	Hatoyama, S	-	1.7–2.6	1.7–2.6	6.5–8.0	6.5–7.5	97.1	H. trueperi
158	Nagareyama, C	-	0.9–1.7	0.9–1.7	6.5–8.0	6.5–7.5	96.4	H. trueperi
108	Iwatsuki, S	Y	1.7–3.4	1.7–2.6	6.5–8.0	6.5–7.5	100	L. salicampi (AY057394)
101	Omiya, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	96.0	P. ryukyuensis (AB087828)
174	Higashimatsuyama, S	Y	0–4.3	0–0.9	5.5–8.0	6.5–7.5	96.0	P. ryukyuensis
177	Kamifukuoka, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	95.8	P. ryukyuensis
198	Kiyose, T	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	95.6	P. ryukyuensis
169	Ageo, S	-	0–4.3	0–0.9	5.5–8.0	6.5–7.5	95.5	P. ryukyuensis
121	Toride, I	P	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	93.5	V. carmonensis (AJ316302)
141	Ranzan S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	99.9	V. halodenitrificans (AB021186)
148	Sakado, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	99.7	V. halodenitrificans
147	Matsubushi, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	99.5	V. halodenitrificans
160	Fujioka, Tg	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	94.4	V. halodenitrificans
137	Urawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	94.1	V. halodenitrificans
201	Omiya, S	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	100	V. marismortui (AJ009793)
126	Tsurugashima, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	98.3	V. marismortui
123	Urawa, S	-	0–3.4	0–0.9	6.5–8.0	6.5–7.5	97.3	V. marismortui
161	Tsurugashima, S	P	0–3.4	0–0.9	6.5–8.0	6.5–7.5	96.9	V. picturae (AJ315060)
138	Urawa, S	P	0.9–1.7	0.9–1.7	6.5–8.0	6.5–7.5	96.7	V. picturae
179	Urawa, S	-	1.7–4.3	1.7–2.6	6.5–8.0	6.5–7.5	95.9	V. picturae
149	Ryugasaki, I	-	0.9–2.6	0.9–1.7	6.5–8.0	6.5–7.5	95.8	V. picturae
134	Kamifukuoka, S	P	0.9–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.4	V. picturae
135	Urawa, S	P	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	95.1	V. picturae
73	Sakado, S	-	1.7–4.3	1.7–2.6	6.5–8.0	6.5–7.5	88.4	B. agaradhaerens (X76445)
219	Kasukabe, S	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	100	B. megaterium (AY553118)
216	Sekijo, I	-	0–1.7	0–0.9	5.5–8.0	6.5–7.5	99.3	H. litoralis (X94558)
214	Sayama, S	-	1.7–2.6	1.7–2.6	5.5–8.0	6.5–7.5	96.3	H. litoralis
211	Higashichichibu, S	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	96.1	H. litoralis
213	Sekijo, I	-	0–1.7	0–0.9	5.5–8.0	6.5–7.5	95.9	H. litoralis
215	Kawagoe, S	-	0–4.3	0.9–1.7	5.5–8.0	6.5–7.5	95.9	H. litoralis
212	Sayama, S	-	0–3.4	0.9–1.7	6.5–8.0	6.5–7.5	94.9	H. litoralis
218	Toride, I	-	0–4.3	0–0.9	6.5–8.0	6.5–7.5	97.5	H. trueperi (AJ310149)
220	Hachioji, T	-	0–3.4	0.9–1.7	5.5–8.0	6.5–7.5	100	V. halodenitrificans (AB021186)
217	Itabashi, T	-	0–3.4	0–0.9	5.5–8.0	6.5–7.5	94.5	V. necropolis (AJ315056)

Strains No. 1–31 isolated on alkaline medium (pH 9.0), strains No. 51–203 isolated on neutral medium (pH 7.0) and strains No. 211–220 isolated on acidic medium (pH 5.0). Abbreviations of prefectures: C, Chiba; G, Gunma; I, Ibaraki; K, Kanagawa; S, Saitama; T, Tokyo; Tg, Tochigi. Abbreviations of pigmentation:–, None; B, Brown; P, Pink; Y, Yellow. Abbreviations of generic names: B., Bacillus; F., Filobacillus; G., Gracilibacillus; H., Halobacillus; L., Lentibacillus; P., Paraliobacillus; Pc., Planococcus; V., Virgibacillus.

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