Figure 3
From: Metaproteomic analysis of Chesapeake Bay microbial communities
The harvesting protocol for microbial communities does not bias against different types of bacteria. Proteomes of Chlorobium tepidum (a), Escherichia coli (b) and Pseudomonas fluorescens (c) and the metaproteomes of an artificially constructed community containing all three organisms (d) were overlain and compared to the metaproteomes of the artificial community after dilution and recovery using Compugen Z3 software. Green or pink colored protein spots are unmatched. Gray or black spots are matched. Total 100 μg proteins are loaded on each polyacrylamide gel and the gels are stained by SYPRO Ruby. pI, isoelectric point; Mr, molecular weight.