Primer paira | Sequence (5'-3')b | Primer binding sitec | Product size (bp) |
---|
RH-halo-1F | GTTCTTcTtGGTACAAGAACAGA | 192–214 | 144 |
RH-halo-1R | GCATGAGTATTCACATCTT | 317–335 | |
RH-halo-1F | GTTCTTcTtGGTACAAGAACAGA | 192–214 | 187 |
RH-halo-2R | GGAATTCCTGTGTCAAgAAaTGA | 356–378 | |
- a. (F) and (R) correspond to forvard and reverse primers, respectively.
- b. Bases that do not match appropriate sequences are shown as lowercase letters.
- c. Positions within the Desulfocella halophila DSM 11763 (AF418200; Friedrich, 2002) dsrA open reading frame.