Figure 2
From: Compatible solute influence on nucleic acids: Many questions but few answers
Solute effects on meting temperature T M . The melting temperature TM of double stranded DNA is shifted by solutes. Compatible solutes like betaine, tetrahydropyrimidines and proline lower TM. Sodium chloride shows the same shift in opposite direction at concentrations tenfold lower. Dyes interacting with dsDNA like SYBR®-green also increase TM at those very low concentrations which are used in realtime applications. The applied standard concentrations are not disclosed by the manufacturer. (Kurz and co-workers, unpublished data for 50% GC-content.).