Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in Oryza sativaL
© Rodríguez et al; licensee BioMed Central Ltd. 2006
Received: 21 March 2006
Accepted: 06 June 2006
Published: 06 June 2006
Salt stress is one of the most serious factors limiting the productivity of rice, the staple diet in many countries. Gibberellic acid has been reported to reduce NaCl-induced growth inhibition in some plants including rice. Most paddy soils have a natural population of Cyanobacteria, prokaryotic photosynthethic microorganisms, which synthesize and liberate plant growth regulators such as gibberellins that could exert a natural beneficial effect on salt stressed rice plants. The aim of this work was to evaluate the effect of the cyanobacterium Scytonema hofmanni extracellular products on the growth of rice seedlings inhibited by NaCl and to compare it with the effect of the gibberellic acid in the same stress condition. Growth (length and weight of the seedlings) and biochemical parameters (5-aminolevulinate dehydratase activity, total free porphyrin and pigments content) were evaluated.
Salt exposure negatively affected all parameters measured, with the exception of chlorophyll. Chlrorophyll concentrations nearly doubled upon exposure to high salt. Gibberellic acid counteracted the effect of salt on the length and dry weight of the shoot, and on carotenoid and chlorophyll b contents. Extracellular products nullified the salt effect on shoot dry weight and carotenoid content; partially counteracted the effect on shoot length (from 54% to 38% decrease), root dry weight (from 59% to 41% decrease) and total free porphyrin (from 31 to 13% decrease); reduced by 35% the salt increase of chlorophyll a; had no effect on root length and chlorophyll b. Gibberellic acid and extracellular products increased 5-aminolevulinate dehydratase activity over the control without salt. When coincident with high salinity, exposure to either EP or GA3, resulted in a reversal of shoot-related responses to salt stress. We propose that Scytonema hofmanni extracellular products may counteract altered hormone homeostasis of rice seedlings under salt stress by producing gibberellin-like plant growth regulators.
Most paddy soils have a natural population of Cyanobacteria. Some representatives of these prokaryotic photosynthethic microorganisms are a potential source of combined nitrogen because they are capable of fixing atmospheric nitrogen . An additional benefit of cyanobacteria is their capacity to synthesize and liberate bioactive substances such as auxins, gibberellins, cytokinins, vitamins, polypeptides, aminoacids, which promote plant growth and development [2–4].
Coastal salinity and accumulation of salts in irrigated land are primary factors depressing yield in rice crop. Salinity can affect germination, metabolism, the size of plants, branching, leaf size and overall plant anatomy. Salt also affects photosynthetic components such as enzymes, chlorophyll and carotenoid contents  as well as the activity of ALA-D , second enzyme of porphyrin biosynthetic pathway that produce heme group substances and chlorophyll in plants. The inhibitory effect of salt stress on plant growth is exhibited at several levels and involves an array of cellular processes such as cell division and expansion. These cellular processes are regulated by hormones for which homeostasis may be altered by salt. Several reports have indicated that application on crops of growth regulators, such as GA3 and cytokinin, produced some benefit in alleviating the adverse effects of salt stress . GA3 reduced NaCl-induced growth inhibition of shoot rice seedlings , and kinetin lightened the influence of salinity on growth and production of plant growth regulators in Vigna sinensis and Zea mays .
The aim of this work was to evaluate the capacity of Scytonema hofmanni EP to counteract the NaCl-induced growth inhibition in rice seedlings and to compare it with the beneficial effect of gibberellic acid.
An axenic strain of the cyanobacterium Scytonema hofmanni (14 a), from the culture collection of the Laboratory of Cyanobacteria, University of Buenos Aires , was selected for this experiment because it had previously showed a promoting effect on the shoot length of rice seedlings in the same way as GA3 (Rodríguez, personal communication). S. hofmanni was cultured in W modified medium , under fluorescent light (45 μmol photon m-2 s-1), photoperiod 12:12. At day 14 the cyanobacterial biomass was separated from the culture medium by centrifugation (40 min, 8000 × g, 10°C) under sterile condition. The productivity was 1.4 mg biomass DW/mL culture. The supernatant (EP) containing extracellular products, was sterilized by 0.22 μm pore size Millipore membrane and storaged at 4°C. Germinated sterile caryopses of Oryza sativa L. cv. Yeruá PA (kindly provided by La Arrocera Argentina SA) were cultivated in hidroponia. Plastic boxes containing 650 mL of the different culture media were placed under fluorescent light (45 μmol photon m-2 s-1), photoperiod 12:12, 31 ± 1°C and 80 percentage humidity. The culture media were: W+5 g NaCl/L; W+5 g NaCl/L +0.5 mg GA3/L; EP+5 g NaCl/L and W (control). The concentration 5 g NaCl/L (85 mM) had been previously proved to inhibit all the parameters studied. We had also established that seedlings were most sensitive to salt at day 14. To avoid any effect of this strain capacity to fix nitrogen on the biological and biochemical parameters, from day 5, all treatments were added with 2 mL per day of the complete mineral solution of Hoagland, containing combined nitrogen . At day 14, seedlings were harvested and shoot length, shoot dry weight (DW), root lenght and root DW were measured and the shoots were used for biochemical determinations, chlorophyll a, b and carotenoids, according to ; total protein, total free porphyrin and ALA-D (E.C. 18.104.22.168) activity according to . One unit of ALA-D activity (U) was defined as the amount of enzyme which catalyzes the formation of 1 nmol of product/h under the standard incubation condition. The values obtained in a completely randomized design were analyzed by a one way-ANOVA and DGC test , using InfoStat statistical program 2002.
GA3 counteracted the inhibition of shoot growth produced by salt (Fig. 1A and 1C). Lin and Kao  also established that salt-induced inhibition of rice shoot growth was reduced by GA3. In our experiment GA3 showed no difference with NaCl in the inhibition of root growth (Fig. 1B). GA3 produced an inhibition on root DW (72% compared with control), which is higher than the inhibition produced by salt (Fig. 1D).
EP partially counteracted NaCl-induced inhibition on shoot length (16% less inhibition) This result coincide with those obtained by inoculating Cyanobacteria in paddy fields affected by salt . EP also increased root DW by 18% the salt value with salt (Fig. 1D). EP nullified the salt effect on shoot DW (Fig. 1C), but had no effect on the root growth inhibition by NaCl (Fig. 1B). GA3 is known to only affect shoot characters. GA3 and EP treatments alleviated the adverse salt effect on the shoot growth but were ineffective with respect to root growth. The coincident biological effects of GA3 and EP only on shoot growth indicate a possibility of EP containing gibberellin-like substances.
Salt exposure negatively affected all biochemical parameters with the exception of chlorophyll. Salt reduced ALA-D activity by 23% (Fig. 1E) This result coincide with other authors who also observed that, besides lowering ALA-D activity salt decreased GA3 content and increased ABA content . Salt also reduced total free porphyrins by 31% (Fig. 1F), and carotenoids by 42% (Fig. 1I) but increased chlorophyll a and b by 83% and 96%, respectively (Fig. 1G and 1H). It was also observed in other rice cultivars that carotenoids were reduced by NaCl [5, 16]. However, in other model systems, such as salt tolerant green algae, chlorophyll:carotenoids ratio tend to decrease as a function of salinity . There are very few data available on the increase of chlorophyll content by salt. Pervaiz et al. demonstrated that chlorophyll content was progressively increased with the salinity in wheat .
GA3 and EP increased ALA-D activity alike, surpassing the control value by about 50% (Fig. 1E). Total free porphyrin was reduced by NaCl+GA3 by 70% comparing with the control without salt, so this parameter resulted in 55% reduction compared to NaCl treatment (Fig. 1F). GA3 did not counteract the effect of salt on chlorophyll a (Fig. 1G), but it did on chlorophyll b (Fig. 1H).
Compared to salt, EP increased total free porphyrin by 18% (Fig. 1F) and compared to the control without salt increased chlorophyll a content (Fig. 1G). With respect to chlorophyll b, EP did not produced a significant difference compared with salt (Fig. 1H). So, GA3 and EP produced similar effects on ALA-D activity and carotenoid content. EP partially reverted salt effect on total free porphyrin and increased chlorophyll a content; GA3 totally reverted the salt effect on the chlorophyll b content taking it to the control value. The decrease of carotenoid content produced by salt was totally counteracted by EP as well as by GA3.
In Lupinus termis, Haroun and Hussein  established that seed priming in cyanobacterial cultures filtrates increased a and b chlorophyll and reduced carotenoids content. Regarding plant growth regulators, this treatment also increased auxin, gibberellic acid and cytokinin content and decreased ABA content. There is a general agreement on the induction of the endogenous hormone levels by exogenous application of different growth regulators . In our experiment the improvement of rice seedling salt tolerance was probably due to the presence of hormones in the EP produced by the cyanobacterium. Treatment with GA3 may have counteracted the excess of ABA produced as a response to the salt stress, reducing alterations in the growth phytoregulators ratios.
S. hofmanni EP reverted completely or partially many of the NaCl-induced effects on growth and also the biochemical alterations of Yerua PA rice seedlings. EP partially coincided with GA3 in the amelioration of salt affected parameters. The cyanobacterial EP would contain gibberellin-like substances which may be responsible of the alleviation of salt stress adverse effect on hormone homeostasis.
However, it would be important to confirm the presence of gibberellins in S. hofmanni extracellular products as well as the alteration of the hormonal homeostasis in this rice cultivar under salt stress.
aminolevulic acid dehidratase
indol acetic acid
Watanabe culture medium
- Mishra U, Pabbi S: Cyanobacteria: A potential biofertilizer for rice. Resonance J Sci Education. 2004, 9: 6-10.Google Scholar
- Zulpa de Caire G, Zaccaro de Mulé MC, Storni de Cano M: Productos extracelulares de Nostoc muscorum Ag. (cepa 79a) obtenidos en medios con y sin nitrogeno combinado. I: sus efectos sobre plántulas de arroz. Int J Exp Bot (Phyton). 1979, 37: 1-13.Google Scholar
- Stirk WA, Ördög V, Van Staden J: Identification of the cytokinin isopentenyladenine in a strain of Arthronema africanum (Cyanobacteria). J Phycol. 1999, 35: 89-92. 10.1046/j.1529-8817.1999.3510089.x.View ArticleGoogle Scholar
- Sergeeva E, Liaimer A, Bergman B: Evidence for production of the phytohormone indole-3-acetic acid by cyanobacteria. Planta. 2002, 215: 229-238. 10.1007/s00425-002-0749-x.View ArticleGoogle Scholar
- Sultana N, Ikeda T, Itoh R: Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environ Exp Bot. 1999, 42: 211-220. 10.1016/S0098-8472(99)00035-0.View ArticleGoogle Scholar
- Younis ME, El-Shahaby OA, Nemay Alla MM, El-Bastawisy ZM: Kinetin alleviates the influence of waterlogging and salinity on groth and affectes the production of plant growth regulators in Vigna sinensis and Zea mays. Agronomie. 2003, 23: 277-285. 10.1051/agro:2003010.View ArticleGoogle Scholar
- Xiong L, Zhu JK: Salt tolerance. The Arabidopsis Book. Edited by: Somerville C, Meyerowitz E. 2002, Rockville MD, American Society of Plant Biologists, 1-22. 10.1199/tab.0048.Google Scholar
- Lin CC, Kao CH: NaCl stress in rice seedlings: starch mobilization and the influence of gibberellic acid on seedling growth. Bot Bull Acad Sin. 1995, 36: 169-173.Google Scholar
- Halperin DR, Storni de Cano M, Zaccaro de Mulé MC, Zulpa de Caire G: Diazotrophic cyanobacteria from Argentine paddy fields. Int J Exp Bot (Phyton). 1992, 53: 135-142.Google Scholar
- Dunn A, Arditti J: Experimental Physiology: experiments in cellular, general, and plant physiology. New York. Edited by: Holt, Rinehart, Winston. 1968, 265-Google Scholar
- Lichtenthaler HK, Wellburn AR: Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans. 1983, 11: 591-592.View ArticleGoogle Scholar
- Stella AM: Estudios sobre la enzima δ-aminolevúlico dehidrasa. PhD Thesis. 1977, Universidad de Buenos Aires, Buenos Aires, ArgentinaGoogle Scholar
- Di Rienzo JA, Guzman AW, Casanoves F: A multiple comparisons method based on the distribution of the root node distance of a binary tree. J Agr Biol Environ Stat. 2002, 7: 1-14. 10.1198/108571102317475017.View ArticleGoogle Scholar
- Zafar S, Ashraf MY, Sarwar G, Mahmood S, Kausar A, Ali I: Variation in growth and ion uptake in salt tolerant and sensitive rice cultivars under NaCl salinity. Asian J Plant Sci. 2004, 3: 156-158.View ArticleGoogle Scholar
- Aziz MA, Hashen MA: Role of Cyanobacteria on yield of rice in saline soil. Pakistan J Biol Sci. 2004, 7: 309-311.View ArticleGoogle Scholar
- Lima MDG, Lopes NF, Bacarin MA, Mendes CR: Efeito do estresse salino sobre a concentracao de pigmentos e prolina em folhas de arroz. Braganti Campinas. 2004, 63: 335-340.View ArticleGoogle Scholar
- Gómez PI, Barriga A, Cifuentes AS, González MA: Effect of salinity on the quantity and quality of carotenoids accumulated by Dunaliella salina (strain CONC-007) and Dunaliella bardawil (strain ATCC 30861) Chlorophyta. Biol Res. 2003, 36: 185-192.View ArticleGoogle Scholar
- Pervaiz Z, Afzal M, Xi S, Xiaoe Y, Ancheng L: Physiological parameters of salt tolerance in wheat. Asian J Plant Sci. 2002, 1: 478-481.View ArticleGoogle Scholar
- Haroun SA, Hussein MH: The promotive effect of algal biofertilizers on growth, protein pattern and some metabolic activities of Lupinus termis plants grown in siliceous soil. Asian J Plant Sci. 2003, 2: 944-951.View ArticleGoogle Scholar
- El-Shahaby OA: Internal water status, endogenous levels of hormones, photosynthetic activity in well watered and previously water stressed Vigna sinensis plants under ABA effect. Mans Sci Bull. 1992, 19: 229-245.Google Scholar
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